Abstract: Abstract: Objective The aim of this study was to compare the different features of hepatitis b mice model which were produced by hydrodynamic transfection in different plasmids． Method Inject two plasmids( pcDNA 3. 1 － 1. 3 － C and HBV PAAV － 1. 2 － HBV) to establish two kinds of hepatitis b mice model by hydrodynamic transfection method，and compare the difference and special effects of the models by inspecting the modeling the success rate ，mouse peripheral blood HBsAg and stability，flow detection such as T cells index analysis． Ｒesult The model success rate of pcDNA3. 1 － 1. 3 － HBV -C plasmid was 78. 6%，and after transfection the content of HBsAg could rise to 220 IU/mL in maximum; while the model success rate of Plasmid PAAV － 1. 2 － HBV -A was 56%，and the highest content of HBsAg after transfection was 2300 IU/mL． The immunohistochemical mice liver biopsy was implied that the mice after the transfection of pcDNA 3. 1 － 1. 3 － C plasmid were darker and had a larger area than the mice after the transfection of PAAV － 1. 2 － HBV -A plasmid． Six weeks after transfection plasmid， the peripheral blood of experimental mice was taken to flow cytometry test，and the results showed that comared to normal mice ， the CD4 + and the CD8 + made no difference between the two kinds of mice by the transfection of two plasmids． Conclusion Building hepatitis b mice model by using the method of hydrodynamic transfection could make a significant difference in hepatitis b virus expression level and stability．

Key words: <p>hydrodynamic Injection, hepatitis b mice model, HBV transfection plasmid</p>

摘要： 摘要: 目的比较不同质粒建立的水动力转染乙型肝炎小鼠模型的特性。方法将pcDNA 3. 1-1. 3-HBV-C 与PAAV-1. 2-HBV-A 两种质粒通过水动力转染法建立两种乙型肝炎小鼠模型，通过对建模成功率和小鼠外周血HBsAg 滴度及稳定性，T 细胞指数流式等检测分析，比较不同模型差异和特效。结果转染pcDNA3. 1-1. 3-HBV-C质粒建模成功率为78. 6%，转染后体内HBsAg 滴度最高达到220IU/mL; 转染PAAV-1. 2-HBV-A 质粒建模成功率为56%，转染后体内HBsAg 滴度最高达到2 300IU/mL。建模小鼠的肝脏进行切片，做免疫组化后，观察实验结果，转染pcDNA 3. 1-1. 3-HBV-C 质粒的小鼠肝脏切片的HBsAg 免疫组化较PAAV-1. 2-HBV-A 质粒小鼠颜色面积更大。实验小鼠转染质粒后6 周的外周血进行流式细胞术检测，结果显示特异性T 细胞CD4 +、CD8 + 和正常小鼠比较，两种质粒的造模小鼠未有差别。结论利用水动力转染法用不同质粒建造乙型肝炎小鼠模型在乙肝病毒指标水平和稳定性方面有着显著差异。

关键词: <, p>, 水动力转染法, 乙型肝炎小鼠模型, 转染HBV 质粒<, /p>