Abstract: Abstract: Objective To find the immunological reactions post H1N1 influenza infection． Method Mice were infected with 0. 1LD50 A/PＲ/8 /34 ( H1N1) influenza viruses． Level of IL-5 /6 cytokines were tested by ELISA，and ratio of effective T cells were analyzed by flow cytometer． Ｒesult The virus loading in mice lung was peaked at 3dpi，and the level of IL-6 in serum was peaked on 5dpi with 122pg /mL． The level of IL-5 in BAFL was peaked at 7 dpi with 680pg /mL，and the ratio of CD4 + CD44 + CD62L-ratio T lymphocytes rised significantly． Conclusion C57BL /6 mice infected with PＲ8 virus is a good model to study immunology reaction of influenza virus infection．

Key words: <p>H1N1 virus, mice infection, IL-5, IL-6</p>

摘要： 摘要: 目的研究H1N1 流感病毒感染能力及机体免疫应答反应。方法利用0. 1LD50剂量A/PＲ/8 /34 ( H1N1) 病毒感染C57BL/6 小鼠，利用ELISA 和流式细胞术等方法检测IL-5、IL-6 水平和CD4 + 效应性T 淋巴细胞比例。结果3dpi 小鼠肺脏病毒复制达到高峰，为107EID50 /g，7dpi 肺脏组织病理学观察呈间质性肺炎、炎性细胞浸润; 5dpi 时IL-6 水平达到峰值，为122pg /mL; 7dpi 时IL-5 达到峰值，为680pg /mL; 与未感染组相比，感染组CD4 + CD44 +CD62L － 效应T 细胞比例显著升高。结论结果表明PＲ8 流感病毒感染小鼠，肺脏病毒复制水平高，机体免疫应答强，适合用于进一步的免疫学研究。

关键词: <, p>, H1N1 病毒, 小鼠感染, IL-5, IL-6<, /p>