Abstract: Abstract: Objective To provide an efficient genetic diagnostics method for Labrador Ｒetrievers，6 SNPs of 5 canine hip dysplasia ( CHD) related genes in genomic DNA of Labrador Ｒetrievers were detected． Method 11 normal and 6 typical CHD Labrador Ｒetrievers were selected by imaging diagnosis． The vein blood was sampled and the genomic DNA was isolated from white blood cells． The PCＲ/sequencing method was used to analyze the variants of 6 SNPs in 5 canine hip dysplasia ( CHD) related genes ( LAMA2 ( CFA1: 70997779) ，LＲＲ1 ( CFA8: 29247021 ) ，SCＲ ( CFA24: 28944481 ) ，COL6A3 ( CFA25: 51031100， 51040259 ) ， and FN1 ( CFA37: 25095511) ) ． Furth more，the association between the SNP variants and CHD was analyzed by Chi-square Test．Ｒesult The variants of LAMA2 ( CFA1: 70997779 ) ，LＲＲ1 ( CFA8: 29247021 ) ，SCＲ ( CFA24: 28944481 ) ，COL6A3 ( CFA25: 51031100) ，and FN1 ( CFA37: 25095511) SNP loci were not significantly associated to CHD ( P ＞ 0. 05) ． A/A variants in COL6A3( CFA25: 51040259) SNP locus was significantly associated to CHD ( P = 0. 007) ． Conclusion A/A variants in COL6A3 ( CFA25: 51040259) SNP locus might be used as a practical genetic detection index for CHD diagnostics in Labrador Ｒetrievers． However，further confirmation in large-sample is required．

Key words: <p>Labrador Ｒetrievers, CHD,  SNP, genetic diagnostics</p>

摘要： 摘要: 目的检测拉布拉多犬基因组DNA 中与髋关节发育不良密切相关的5 个基因中的6 个SNP 位点，以期为拉布拉多犬的选育提供有效的遗传学诊断方法。方法经影像学诊断，筛选出典型的6 只髋关节发育不良和11 只 正常拉布拉多犬作为实验对象，采取静脉血，提取血细胞的基因组DNA 样本，采用PCＲ/测序方法对17 个样本的5个基因中的6 个SNP 位点: LAMA2( CFA1: 70997779) ，LＲＲ1( CFA8: 29247021) ，SCＲ( CFA24: 28944481) ，COL6A3( CFA25: 51031100， 51040259) 和FN1( CFA37: 25095511) 进行变异型检测，并统计分析SNP 位点的变异型与髋关节发育不良犬关联度。结果LAMA2( CFA1: 70997779) ，LＲＲ1( CFA8: 29247021) ，SCＲ( CFA24: 28944481) ，COL6A3( CFA25: 51031100) 和FN1( CFA37: 25095511) SNP 位点的变异型与拉布拉多犬的髋关节发育不良没有显著相关性( P ＞ 0. 05) 。COL6A3( CFA25: 51040259) SNP 位点的A/A 型与拉布拉多犬的髋关节发育不良存在极显著相关性( P = 0. 007) 。结论COL6A3( CFA25: 51040259) SNP 位点的A/A 型可能成为拉布拉多犬髋关节发育不良的实用性遗传学诊断指标，但尚需大样本确认。

关键词: <, p>, 拉布拉多犬, 髋关节发育不良( CHD) , 单核苷酸多态性( SNP) , 遗传学诊断<, /p>