Abstract: Abstract:Objective To introduce a new method of intraperitoneal injection in rat anesthetization to decrease the anesthesia mortality． Method One hundred SD rats were divided into 2 groups: control group and experimental group． The animals were anesthetized by intermittent intraperitoneal injection in experimental group，while the control group anesthetized by single injection． Differences in the anesthetic onset timespan，duration and mortality were assessed between two groups． Ｒesult In control group，the rats with the onset time of 3. 8 ± 1. 4 minutes， duration 115. 5 ± 12. 8 minutes and anesthesia mortality was 12% ; in experimental group，onset time of 10. 9 ± 2. 5 minutes，duration 129. 3 ± 9. 8 minutes and zero anesthesia mortality． Conclusion Intermittent intraperitoneal injection in rat anesthetization could decrease the anesthesia mortality and improve the animal surgery success ratio．

Key words: rats, chloral hydrate, anesthesia, mortality

摘要： 摘要:目的介绍一种大鼠腹腔麻醉的改进方法，提高大鼠麻醉存活率。方法取100 只SD 大鼠随机分为2 组， 每组50 只大鼠，对照组采用传统的一次性腹腔注射，实验组采用改进的间断腹腔注射。分别比较两组麻醉起效时 间、麻醉维持时间、麻醉药浓度可控性及术中及术后死亡率等。结果对照组麻醉大鼠的起效时间(3. 8 ± 1. 4) min，维持时间(115. 5 ± 12. 8)min，死亡率为12% ;实验组麻醉大鼠的起效时间(10. 9 ± 2. 5)min，维持时间(129. 3 ± 9. 8)min，死亡率为0。结论采用间断腹腔注射法进行麻醉可降低大鼠麻醉死亡率，提高动物手术的成功率。

关键词: 大鼠, 水合氯醛, 麻醉, 死亡率