Abstract: Abstract: Objective To observe the effect of Kv1. 2、Kv3. 4 channels in rat pulmonary arterial smooth cells on the process of pulmonary vasoconstriction induced by 15 － KETE． Method Western blot and PCR were used to identify the effect of 15 － KETE on expression of Kv1. 2、Kv3. 4 channels in cultured rat pulmonary arterial smooth muscle cells ( PASMCs) ． Result ( 1 ) The expressions of Kv1. 2 mRNA and protein in rat PASMCs were significantly down-regulated by 15 － KETE． ( 2) The expressions of Kv3. 4 mRNA and protein in rat PASMCs were significantly down-regulated by 15 － KETE． Conclusion Kv1. 2、Kv3. 4 channels are involved in vasoconstriction induced by 15 － KETE in rat PASMCs．

Key words: 15 － ketoeicosatetretraenoic acid, hypoxic pulmonary vasoconstriction, voltage-gated potassium channelpulmonary arterial smooth cells

摘要： 摘要: 目的从细胞分子水平研究电压门控型钾通道亚型Kv1. 2、Kv3. 4 在15 － 酮基二十碳四烯酸( 15 － ketoeicosatetretraenoic acid， 15 － KETE) 致大鼠肺动脉收缩过程中的作用。方法通过酶法分离、培养SD 大鼠肺动 脉平滑肌细胞( pulmonary arterial smooth cells，PASMCs) ，使用RT-PCR 和Western blot 技术观察15 － KETE 对大鼠 PASMCs 上Kv1. 2、Kv3. 4 通道表达的影响。结果( 1) 15 － KETE 下调大鼠PASMCs 膜上Kv1. 2 通道mRNA 及蛋白 质的表达; ( 2) 15 － KETE 下调大鼠PASMCs 膜上Kv3. 4 通道mRNA 及蛋白质的表达。结论Kv1. 2、Kv3. 4 通道参 与由15 － KETE 引起的缺氧肺动脉收缩( hypoxic pulmonary vasoconstriction ，HPV) 。

关键词: 15 － 酮基二十碳四烯酸, 缺氧肺动脉收缩, 电压门控性K + 通道亚型, 肺动脉平滑肌细胞