Abstract: Abstract: Objective To observe infectiousness of Echinococcus multilocularis by different inoculation methods in Cricetulus migratorius so as to provide the foundation for developing hydatid disease animal model in variety ofcavity，heart or liver． The development status and parasitic parts of the cysts were surveyed by dissection，and cysts coefficient ( cyst weight / body weight × 100% ) and infection rates were computed． Result The infection rate in 29 animals was 100% with a cyst coefficient of 7. 1% at 100 days after intraperitoneal inoculation． The cyst parasitism in the abdominal cavity was found in all 7 sacrificed animals，and liver cyst infiltration was found in 3 animals，spleen cyst infiltration in 2 animals and kidney cyst infiltration in 2 animals with total cyst coefficient of 51. 7% at 300 days after intraperitoneal inoculation． At 116 days after armpit inoculation，the cyst parasitism in armpit was revealed in all 19 sacrificed animals with a cyst coefficient of 3. 27% ，and at 290 days after armpit inoculation the cyst parasitism in armpit was revealed in all 5 sacrificed animals with a cyst coefficient of 22. 95% ． At 100 days after liver inoculation in 17 animals，the cyst parasitism in the abdominal cavity revealed in 8 animals， the cyst parasitism in liver in 5 animals，the cyst parasitism in heart in 1 animal，and the cyst parasitism in lung in 3 animals with a cyst coefficient of 7. 1% ． At 115 days after heart inoculation in 26 animals，the cyst parasitism in heart revealed in 6 animals，the cyst parasitism in lung in 9 animals，and the cyst parasitism in thorax in 1 animal with a cyst coefficient of 2. 91% ． Conclusion The celiac environment of Cricetulus migratorius may be hospitable for the growth and development of Em，which is appropriate for developing of the short-period animal model of hydatid disease． Em grows and develops slowly in the forelimb armpit of Cricetulus migratorius，so that the armpit inoculation is suitable for bread conservation of Em． Because of the lower in fecting rate for inoculating heart and liver in the animal models，the inoculation technology and methods should be improve，so as to increase the infection．

Key words: Echinococcus multilocularis ( Em), Cricetulus migratorius, animal models, cysts coefficient

摘要： 摘要: 目的观察将多房棘球蚴( Echinococcus muhilocularis 简称Em) 接种灰仓鼠腹腔、前肢腋下、心脏和肝脏组织 的感染效果，为探索建立多组织或器官包虫病动物模型提供依据。方法按1000 个头节/只剂量经腹腔和前肢腋 下接种灰仓鼠，经腹膜穿刺肝脏和经胸腔穿刺心脏接种灰仓鼠，按计划解剖检查包囊发育状况和包囊寄生部位，计 算包囊系数( 包囊质量/ 体质量× 100% ) 和感染率。结果100 d 处理腹腔接种29 只，腹腔100% 感染，包囊系数 7. 1%，300 d 处理7 只，除腹腔都有包囊寄生外，包囊浸润肝脏3 只，浸润脾脏和肾脏各2 只，总包囊系数51. 7% 。 前肢腋下接种的分别于116 d 和290 d 处理19 只和5 只，腋下均有包囊寄生，包囊系数分别为3. 27% 和22. 95% 。 100d 处理肝脏接种的17 只，共10 只有包囊，包囊系数7. 1% ，包囊寄生部位为腹腔8 只，肝脏5 只，心脏1 只，肺部 3 只。115 d 处理心脏接种26 只，包囊寄生部位为心脏6 只，肺部9 只，胸腔1 只，其中有两只同时心脏和肺脏寄生 包囊，共13 只寄生包囊，包囊系数2. 91% 。结论腹腔环境适宜Em 生长发育，适合做周期短的动物模型，接种 300 d 包囊有向实体器官浸润的现象。Em 在前肢腋下发育缓慢，适合做Em 保种。由于动物模型中的心脏和肝脏 接种感染率低，接种技术和方法需改进，以便提高感染率． 关键词: 多房棘球蚴( Em) ; 灰仓鼠; 动物模型; 包囊系数; 器官

关键词: 多房棘球蚴( Em), 灰仓鼠, 动物模型, 包囊系数, 器官