Abstract: Abstract: Objective To estimate the acute toxicity of crude polysaccharide extracted from juglans mandshurica maxim to mice． Method Crude polysaccharide extracted from juglans mandshurica maxim ( concentration of 5. 4 mg /mL) was orally administrated to the mice at a dose of 40 mL / kg，three times per day，and was injected intraperitoneally to the mice at a dose of 20 mL / kg，two times per day． After administrated for 1 days，continuous observated 7 days． Result In the course of the test，the activity and diet of mice were normal，and there were no death of the mice． Autopsy revealed nothing remarkable． The maximum dosage of oral administration and the maximum dosage of intraperitoneal injection were 6. 48mg /10g and 2. 16 mg /10g respectively． Conclusion Toxic effect of the rude polysaccharide extracted from juglans mandshurica maxim on the mice in the two administration routes was low．  crude polysaccharide extracted from juglans mandshurica maxim; mice; intragastric administration; intraperitoneal injection

Key words:  crude polysaccharide extracted from juglans mandshurica maxim, mice, intragastric administration, intraperitoneal injection

摘要： 40 mL / kg每天3 次灌胃，20 mL / kg 每天2 次腹腔注射，给药1 d 后，连续观察7 d。结果实验期间，小鼠的活动与 饮食情况未见异常，未出现死亡。尸检各脏器无异常。小鼠的核桃楸皮粗多糖每天最大给药量: 腹腔注射 2. 16 mg /10 g，灌胃6. 48 mg /10 g。结论通过两种给药方法证明，核桃楸皮粗多糖对小鼠的毒性作用较低。

关键词: 核桃楸皮粗多糖, 小鼠, 灌胃, 腹腔注射