Abstract: Abstract: Objective To analyse the difference of hematology and serum biochemistry results between PLC ( knockout homozygotic type( － / － ) and wild type( + / + ) mice． Methods 20 PLC( knockout homozygotic type ( － / － ) and 20 wild type( + / + ) mice were selected，to measure the 8 clinical normal values of hematology and 11 clinical normal values of serum biochemistry，and to make contrast analysis between the male and female and between different genotype． Results WBC、RBC、HCT、MCH in wild type( + / + ) and PLT in homozygotic type ( － / － ) mice between female and male had significant difference ( P ＜ 0. 05 ) ; and MCV and MCH of wild type ( + / + ) mice were higher than homozygotic type( － / － ) ( P ＜ 0. 05) ; ALB、GLU、TC in wild type( + / + ) and ALP、TG in homozygotic type( － / － ) mice between female and male had significant difference( P ＜ 0. 05) ; and TBIL of wild type( + / + ) mice were lower than homozygotic type ( － / － ) mice ( P ＜ 0. 05 ) ． Conclusion The quantity( 7) of hematology and serum biochemistry results having difference between female and male in wild type ( + / + ) mice was more than that( 3) of homozygotic type( － / － ) mice． 2 hematology and 1 serum biochemistry results had significant difference between wild type( + / + ) and homozygotic type( － / － ) mice．

Key words: PLCε, gene knock-out, hematolo, serum biochemistry

摘要： 摘要: 目的分析PLCε 基因敲除小鼠纯合型( － / － ) 和野生型小鼠( + / + ) 血液生理生化指标的差异。方法选 取成年PLCε 基因敲除纯合型( － / － ) 小鼠与野生型( + / + ) 小鼠各20 只，雌雄各半，检测8 项生理指标和11 项血 清生化指标。结果野生型( + / + ) 小鼠的WBC、RBC、HCT 和MCH 等4 项生理指标，纯合型小鼠( － / － ) 的PLT 指标，雌雄之间差异显著( P ＜ 0. 05) ; 野生型( + / + ) 的MCV 和MCH 指标高于纯合型( － / － ) 小鼠，差异显著( P ＜ 0. 05) ; 野生型( + / + ) 小鼠的ALB、GLU 和TC 等3 项生化指标，纯合型( － / － ) 小鼠ALP 和TG 等2 项生化指标， 雌雄之间差异显著( P ＜ 0. 05) ; 野生型( + / + ) 小鼠的TBIL 指标低于纯合型( － / － ) 小鼠，差异显著( P ＜ 0. 05) 。 结论野生型雌雄之间有7 项生理生化指标存在差异比纯合型3 项差异指标多，野生型与纯合型之间有2 项血液 生理指标和1 项血清生化指标存在显著差异。

关键词:  , PLC&epsilon, 基因敲除, 血液学, 血清生化