Abstract: Abstract: Objective To find the method of genetic monitoring on mouse microsatellite DNA polymorphisms by select the microsatellite DNA polymorphism primer and optimize the Tm for the inbred mouse strains． Methods Analysis of microsatellite DNA polymorphisms and genetic monitoring of seven inbred mouse strains including C57BL /6、C3H、BALB / c、DBA /2、129、FVB and SCID were investigated by polymerism chain reaction( PCR) with 34 pairs of microsatellite primers loci on different chromosomes of mouse． Results 32 pairs of microsatellite primers could be amplified stably，of which 9pairs of primers display monomorphic among the various inbred strain， 25 pairs of primers display polymorphisms among the different strains，8 pairs of primers had more significant polymorphism． Conclusion Microsatellite DNA displays significant polymorphism，which could be used for various inbred strains identification and genetic monitoring．

Key words: inbred mouse, microsatellite DNA primer, Tm value

摘要： 摘要: 目的通过对近交系小鼠微卫星引物的筛选和Tm 值优化研究，以探索小鼠DNA 多态性检测方法。方法 随机选用32 对位于小鼠不同染色体的微卫星引物，用PCR 扩增方法对常用C57BL /6、C3H、BALB / c、DBA /2、129、 FVB 及SCID 近交系小鼠DNA 多态性进行扩增和电泳分析，并对其中25 对引物的Tm 值进行优化。结果25 对引 物可稳定扩增，2对引物在不同品系小鼠间表现为单态性，23 对引物在不同品系间呈多态性，10 对引物呈显著多态 性( 3 ～ 4 个态性) 。结论所筛选和优化的25 对微卫星引物，对不同品系小鼠DNA 可稳定扩增，其电泳结果具有 较高的DNA 多态性，可反映不同品系小鼠的遗传概貌。

关键词: 近交系小鼠, 微卫星引物, Tm 值