Abstract: Abstract：Objective To explore and summary successful points for establish Langendorff heart perfusion models． Methods Through 1 6 healthy SD rats(200-4009 each)，using HTK solution for eardioplegia and establishingstandard Langendorff heart perfusion models，and compare myocardial enzymes and hemodynamic with continuous perfusion and beating group．Results 1 case failed because of unskilled operation in extracting the heart；1 case failed because of inserting the perfusion—tube too deeply to damage the aortic valve；1 case failed because of lowly indoor-temperature；1 else failed because of excessive diameter of perfusion—tube：1 2 cases prepare successfully with the application of indoor heaters，perfusion needles with different diameters，self—made balloons and accelerating the operations．There’s no significant difference between cardiac resuscitation group and continuous heaa beat group in myocardial enzymes and hemodynamic．Conclusion After mastering the attentions above，isolated heart perfusion models can be established successfully and quickly．

Key words: isolated heart, perfusion model, establish

摘要： 摘要：目的探讨并总结制作Langendorff离体心脏灌注模型的要点及成功经验。方法通过16只健康sD大鼠(体 质量200—400 g)，以HTK液作为心脏停搏液建立标准的Langendorff离体心脏灌注模型。将此模型与持续K—H液心 肌灌注、持续心脏搏动组大鼠的同一时段的心肌酶学及血流动力学指标进行对比，使用SPSS 11．5作统计学霞复测量 资料的方差分析。结果l例凶操作不熟练致提取心脏时间延长，l例因灌注管插人深度过深导致主动脉瓣毁损。 1例凶室温过低，l例因灌注管管径过大无法插入而导致实验失败，其余12例在应用了室内加热器、不同管径灌注针、 自制球囊测压管及加快操作速度后均获得实验成功。所制模型心肌酶学及血流动力学各指标与持续心脏搏动组无 明显差异(P>0．05)。结论掌握制作要点及注意事项后，Langendofff离体心脏灌注模型可顺利快速地成功制备。

关键词: 离体心脏, 灌注模型, 制备