Abstract: Objective In this study，pregnant mare serum gonadotropin(PMSG)and human chorionic gonadotrophin(hCG)were used to make treatments of superovulation with different strains and ages of mice， meanwhile to obtain a better way for superovulation，the dose of hormones(PMSG／hCG)，mouse strains and weeks on the effect of superovulation were compared．Results (1)the hormone doses(with 10 IU)were better than those with 5 IU，but no significant difference wa$observed，(2)when the mice were injected with determined dosage of hormone，the effect of supemvulation with IC R mice and C57 BL／6J mice were significantly better than the BALB／c mice and FVB mice(尸<0．05)，(3)meanwhile，the effect of mice with 8 weeks were better than those with 5 weeks without significance(P>0．05)．Conclusion In this laboratory，using the hormone doses of lO IU PMSG 4-10 IU hCG to 8 weeks old ICR．C57BL／6J were relatively good effect of superovulation．

Key words: supemvulation, dosage, hormone, age, strain, mice

摘要： 目的采用孕马血清促性腺激素(PMSG)和人促绒毛膜性腺激素(hCG)对不同品系和周龄小鼠进行超数排 卵处理，比较激素(PMSG／hCG)注射剂量、小鼠品系和周龄对超排效果的影响，以便获得比较优良的超排方法。结 果I)激素剂量为PMSG(10 IU／只)+hCG(10 IU／只)的超排效果优于PMSG(5 IU／只)+hCG(5 IU／只)．但无 显著差异(P>0．05)；2)相同剂量时。ICR小鼠和C57BL／6J小鼠的超排效果显著优于BALB／e小鼠和FVB小鼠 (P<0．05)；3)5周龄与8周龄的小鼠比较，8周龄超排效果较好．但差异不显著(P>0．05)。结论采用lO IU PMSG+IO IU hCG的组合对8周龄的ICR、C57BL／6J进行超排处理效果相对较好。

关键词: 超数排卵, 剂量, 激素, 周龄, 品系, 小鼠