Abstract: Abstract：Objective The aim of this study was to evaluate the ureteral motility of rats in vivo and to determine the changes in density and morphology of interstitial cells of Cajal(ICC)一like ceils(ICC—LC)at the ureteropelvie junction(UPJ)of rats after artificial distal ureteral obstruction．Then，lhe correlationship was assessed between these two aspects．Methods Of a total of 35 rats，5 served a8 controls(C)，5 underwent sham—operations(SH) and 25 were in the study(S)groups．The left ureters of the rats in the S groups were tied with 5-zero silk ligature approximately 3 mm proximal to the ureterovesical junction(U VJ)．The ureteral motility of rats was measured by external pressure transducers．The UPJs were extracted initially in the C and SH groups，and 3，7，14，28，56 days after ligation of the distal ureter in the study groups(S1，S2，S3，S4 and S5，respectively)．The sections stained by c-kit anticore were studied under a light microscope．Results Ureterohydronephrosis wilt8 observed in all the rats after the obstruction was established．The mean number of ureteral peristaltic rate was(1 9．6±4．037)／ min in C，(18．8±2．168)／min in S，(4．6±1．140)／min in S1，(8．0±2．345)／rain in S2，(5．4±1．673)／ min in S3，(5．4±1．517／min in s4，and(6．2 4-1．789)／min S5．There was a statistically significant decrease in ureteral peristaltic rate in the study groups compared to the C and SH groups．The mean number of ICC—LC was (7．20±2．29)／HP in C，(7．02±2．68)／HP in SH，(3．54±1．85)／HP in S1，(6．30 4-1．19)／HP in S2， (4．30 4-0．67)／HP in S3，(4．42±1．45)／HP in s4，and(4．70±1．09)／HP in S5．There w鹳a statistically significant decrease in ICC·LC density in the study groups，except S2，compared to the C and SH groups．No significant difference was detected in Cajal cell morphology between groups．Conclusion Based on the changes in density of ICC-LC at the UPJ after obstruction of the distal ureter．we suggest that ICC-LC have a close relationship with motility of the ureter．

Key words: ICC-LC, uretersl obstruction, uretersl motility, CDl 17

摘要： 化规律，并分析两者的相关性。方法清洁级SD大鼠35只，随机分为7组，每组5只。大鼠被分为3个大组，5只 作为正常对照组(c)，5只作为假手术组(S)．其余作为实验组(sI—S5)：梗阻3 d组Sl、梗阻l周组s2、梗阻2周组 S3、梗阻4周组S4、梗阻8周组s5。实验组动物建立左侧输尿管完全梗阻模型。外接压力感受器法测定各组大鼠 输尿管蠕动活性．分析其变化规律。取实验侧肾盂输尿管交界处上下各O．2 cm输尿管．采用免疫组织化学显色． 光镜下计数ICC样细胞密度并观察形态。结果所有实验组大鼠左肾盂及输尿管均见明显扩张积水，动物模型建 立成功。各组输尿管蠕动频率分别为：C(19．6±4．037)次／rain．S(18．8±2．168)次／min．SI(4．6±1．140)次／min， s2(8．0±2．345)次／rain。S3(5．4±1．673)次／min。S4(5．4±1．517)次／min，s5(6．2±1．789)次／min，但输尿管蠕动 频率在正常对照组(c)和假手术组(S)问没有显著性差异(P=0．706)。各实验组输尿管蠕动频率较正常对照组 (C)和假手术组(s)有所下降．但呈现出先下降后上升再下降的趋势．这种差异除了在Sl和s2组之间有统计学差 异外，在其他各个实验组之间没有统计学差异。各组ICC样问质细胞的密度分别为：c(7．20：It 2．29)个／视野．S (7．02±2．68)个／视野．Sl(3．54±1．85)个／视野。S2(6．30±I．19)个／视野．S3(4．30±0．67)个／视野．s4(4．42± 1．45)个／视野，s5(4．70±1．∞)个／视野。除了s2组以外的各实验组ICC样间质细胞的密度较正常对照组(c)和 假手术组(s)有所下降，但呈现出先下降后上升再下降的趋势．这种差异在各个实验组之间没有显著的统计学差 异。大鼠输尿管蠕动频率变化趋势与ICC样问质细胞的变化趋势有很好的相关性。绪论大鼠肾盂输尿管交界 处ICC样间质细胞的密度变化与输尿管蠕动活性关系密切，为将来研究ICC样问质细胞在泌尿系统中的作用奠定 了一定的基础。

关键词: ICC样间质细胞, 输尿管梗阻, 输尿管蠕动, CDll7