Abstract: Abstract：Objective To develop the reference values for serum resistin in rhesus monkeys at various ages using ELISA，a rapid quantitative method．Methods The rhesus monkey were divided into three groups(young，adult and elder groups，10 each．group)．Sera were collected and resistin．was detefinined using human—resistin ELISA quantitative detection kit．The OD450 values were analyzed b．y statistic methods。Results The OD450 vslue$ decreased with aging．The mean value was(6 510±1 730)pg／mL in young group，(5 880±l 025)pg／mL in aduh group and(1 760±920)pg／mL in elder group．The value of elder group was significant lower than that of the young group(P<0．0 1)．Conclusion The human—resistin ELISA kit is a quantitative and rapid detection tool for resistin determination．’nle reference values of serum resistin in rhesus monkeys are developed．and they decreased with aging．

Key words: Rhesus monkey, Serum resistin, ELISA

摘要： 目的用ELISA检测试剂盒，探索一种恒河猴血清抵抗素(Resistin)定量的快速检测方法，初步建立各年龄 段恒河猴血清抵抗素的正常参考值。方法选择用于人抵抗素检测的ELISA定量检测试剂盒，对恒河猴血清进行 抵抗紊的测定；按年龄段将实验猴分组，幼年组、成年组和老年组，每组lO只，制备血清，检测抵抗素，并进行统计 分析，初步建立各年龄段恒河猴血清抵抗素的正常参考值。结果用ELISA定量检测试剂盒测定恒河猴血清抵抗 素数值稳定，幼年组、成年组和老年组的血清抵抗素正常参考值分别为(6 510±l 730)pg／mL、(5 880±1 250)pg／ mL和(1 760±920)pg／mL，有随年龄增长而逐步降低的趋势，老年组极显著低于幼年组和成年组(P<0．01)。结 论人抵抗素ELISA检测试剂盒可以作为一种对恒河猴血清抵抗素定量的临时快速的检测方法，且随着年龄的增 长其血清抵抗素正常参考值有逐步降低趋势。

关键词: 恒河猴, 血清抵抗素, ELISA