Abstract: Objective This study was to explore the method to breed and identify complement 3 gene knockout mice．Methods The heterozygote of complement 3 gene knockout mice(C3+／一)were bred and reproduced． Wild genotype(C3+／4-)，heterozygote genotype(C3+／一)，and homozygote genotype(C3一／一)would appear in offspring．The genotypes were characterized using ELISA and PCR．Result The heterozygote mice bred 1 35 offspring．38 mice are wild type，68 mice are heterozygote，and 29 mice are homozygote．Byx2text，tlle segregate ratio of offspring was fitted to the Mendelian ratio．Conclusion Appropriate methods for breeding，reproducing，and identifying are the effective way for acquiring complement 3 gene knockout mice from heterozygote．

Key words: Complement 3, Gene knockout mice, PCR, ELISA

摘要： 目的探讨繁殖和鉴定补体C3基因敲除小鼠的实验方法。方法将所引进的补体C3基因敲除杂合子小鼠 (C3+／一)进行饲养并繁殖，其子代出现三种基因型的小鼠。即纯合子C3一／一、杂合子c3+／一、野生型c3+／+， 采用ELISA与PCR相结合对子代小鼠基因型进行鉴定。结果繁育出135只子代小鼠，经鉴定，o+／+、a+／一、 c3一／一各为38只、68只、29只，经x2检验，子代小鼠分离比例符合盂德尔遗传规律。结论正确的饲养繁殖以及 子代鉴定是从杂合子小鼠中获得补体C3基因敲除小鼠的有效途径。

关键词: 补体C3, 基因敲除小鼠, PCR, ELISA