Abstract:  Objective To explore heterozygote of 8 biochemical markers of inbred mice in order to monitor correctly and efficiently the genetic quality of inbred mice．Methods Filial generation 1 CCBFl were obmined by intercrossing of CBA／Ca and BALB／c；and filial generation 1 B6CBFl were also obtained by intercrossing of CBA／ Ca and C57BIJ6．Then CCBFI and B6CBFl were monitored by biochemical markers．Results Heterozygote of 8 biochemical markers of inbred mice was obtained．Conclusion Alleles of 8 biochemical markers are co-dominant， and their heterozygote maps can be criterion of monitoring the genetic quality of inbred mice．

Key words: Mice, Biochemical marker, Inbred strain

摘要： 目的为了更加准确有效地监测近交系小鼠的遗传质量，对近交系小鼠的8个遗传生化标记杂合位点进行 研究。方法将近交系小鼠CBA／Ca与BALB／c交配得到杂交Fl代动物CCBFI，同时将近交系小鼠CBA／Ca与 C57BL／6交配得到杂交Fl代动物B6CBFI，对CCBFI和BbCBFI进行遗传生化标记检测。结果找到近交系小鼠 8个遗传生化标记的杂合位点。结论近交系小鼠的8个遗传生化标记位点相对应的等位基因均为共显性。杂合 位点的图谱可以作为监测近交系小鼠质量的判定标准。

关键词: 小鼠, 生化标记, 近交系