Abstract: Abstract： Objective To investigate the role of neutrophil elastase inhibitor(Elafin)in pathogenesis of hepatic ischemia—reperfusion injury(HIRI)．Methods The rat model of hepatic ischemia—reperfusion was administrated with Elafin at different doses，and then the levels of ALT，AST and LDH in 8ernm were measured．Meanwhile， pathohistological changes in liver were examined． Results The rats showed severe hepatocytic damage after hepatic ischemia-reperfusion and serum levels of ALT，AST and LDH were significantly raised(P<0．001)． However，those symptoms could be relieved to some extent by preoperative injection of Elafin．Conclusion Neutrophil elastase inhibitor Elafin has a protective effect against hepatic ischemia—reperfusion injury in rats．

Key words: Hepatic iscbemia-reperfusion injury, Neutrophil elastase inhibitor, ALT, AST, LDH

摘要： 摘要：目的探讨重组人Elafin对大鼠肝脏缺血再灌注损伤的保护作用。方法建立大鼠肝缺血再灌注损伤模型， 给予不同浓度的重组人Elafin对其进行治疗，检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)及乳酸脱氢酶 (LDH)含量，并对肝脏病理组织学改变进行观察。结果缺血再灌注后大鼠肝组织损害较重，血清ALT、AST及 LDH水平明显升高(P<0．001)，若手术前给予大鼠一定浓度的重组人Elafin可对肝脏缺血再灌注损伤起到一定的 保护作用。结论重组人Elafin对肝脏缺血再灌注损伤有一定的保护作用。

关键词: 肝缺血再灌注损伤, 重组人Elafin, 谷丙转氨酶(ALT), 谷草转氨酶(AST), 乳酸脱氢酶(LDH)