Abstract: Objective To identify the cause of mass disease in rabbit.Methods Pathological autopy,bacteria isolation and culture,biochemical identification,serum agglutination test,PCR detecting and drug sensitivity test of the isolated strain were conducted on diseased rabbit.Results Combined the results obtained from pathological autopy,bacteria isolation and culture,observation of culture characteristics and morphologic structure,biochemical testing,serum agglutination test and PCR detecting,the disease was diagnosed as Staphylocosis by Staphlococcus aureus.The results of drug sensitivity test showed that the isolated strain was sensitive to Penicillin,ampicillin and vancomycin.However,its resistence to ceftazidime and nitrofurantoin were produced.Conclusion The results of this study provided a basis for the identification of pathogen Staphlococcus aureus and diagnosis of Staphylocosis.

Key words: Rabbit, Staphylococcus aureus, Diagnosis, Drug sensitivity test

摘要： 目的对家兔发病死亡的原因进行诊断。方法对病死家兔进行剖检观察,并进行细菌分离培养与生化鉴定、PCR检测和药敏试验。结果通过对病死家兔进行剖检观察、细菌分离培养、生化鉴定、血清学试验、PCR检测和药敏试验,确诊病死家兔是金黄色葡萄球菌感染引起的细菌性疾病。药敏试验结果表明,分离菌株对青霉素、氨苄西林、万古霉素等抗生素敏感,而对头孢他啶、呋喃妥因已产生耐药性。结论为金黄色葡萄球菌病原鉴定与疾病诊断提供了科学依据。

关键词: 家兔, 金黄色葡萄球菌, 诊断, 药敏试验