Abstract: Objective To isolate and identify Corynebacterium kutscheri from rat,and to provide some useful reference for microbiological monitoring in laboratory animal.Method Pathogenic changes of rat was examined.Bacteria cultivation,biological trial,serum agglutination test and animal experiment were carried out.Results Histological examination of C.kutscheri infected rat demonstrated the organism within lung and liver tissues.Isolation and identification of C.kutscheri were performed from laboratory rat which was killed by C.kutscheri infection during a long-term toxicologic study in Beijing.Conclusion The long-term toxicologic study decreased laboratory rat resistance in stressed,which evoked inapparent infection and cause C.kutscheri infected rat dead.We should recognize the importance of C.kutscheri as a potential pathogen in such state.

Key words: Rat, Corynebacterium kutscher(C.kutscheri), Isolate, Identify

摘要： 目的分离并鉴定了一株鼠棒状杆菌,为实验动物微生物监测提供了有效的参考依据。方法采用病理剖检、细菌学检查等方法,对长期毒性试验中发病大鼠分离菌株进行初步鉴定,应用生物学性状检测、血清凝集试验、动物试验等技术对分离菌株作进一步鉴定。结果从发病大鼠中分离出一株细菌,通过生化特性鉴定,并结合血清学诊断方法和动物试验,确证该大鼠分离菌株为鼠棒状杆菌。结论因长期毒性试验的应激致使动物机体的免疫力下降,可使隐性感染的鼠棒状杆菌引发疾病,导致大鼠死亡,严重影响科研工作,应引起足够的重视。

关键词: 大鼠, 鼠棒状杆菌, 分离, 鉴定