Abstract: With the single cell cloning technique, we cloned H2D8 subline from H 22 cell line stored by China Center for Type Culture Collection in Wuhan (Wuhan H 22 ). Then the comparative analysis of Wuhan H 22 and H2D8 about their dependence on serum when cultured in vitro , growth feature in vivo , pathomorphism and therapeutic feature to CTX were carried on. With the results, we found that there was no significant difference between H2D8 and Wuhan H 22 about the dependence on serum, growth feature in vivo and pathomorphism. When the mice bearing tumor were treated with CTX,the restrain neoplastic rates of H2D8 was higher than that of Wuhan H 22 . It means that H2D8 clonal cell line retained the characteristics of Wuhan H 22 cell lines about the dependence on serum when cultured in vitro , grouwth feature in vivo and pathomorphism. H2D8 has higher sensitivity on the chemotherapy feature to CTX than that of Wuhan H 22 .

Key words: Murine Hepatoma 22, Biological characteristic, Chemotherapy

摘要： 用细胞克隆的方法获得武汉大学典型培养物保藏中心保存的小鼠肝癌H2 2 瘤株 (武汉H2 2 )的克隆细胞株H2D8。比较武汉H2 2 及H2D8对血清的依赖性、致瘤性、病理特征和对环磷酰胺 (CTX)的敏感性。结果发现H2D8细胞株对血清的依赖性 ,和对小鼠致瘤性及肿瘤病理学特性与武汉H2 2 细胞无明显差异 ;H2D8细胞对CTX治疗的敏感性更高。说明H2D8克隆株在体外培养、致瘤性和病理特征方面能保持原瘤株的细胞特性 ;在化疗敏感性方面优于原代细胞

关键词: 小鼠肝瘤H22