Abstract: objective:to observe the stability of replication and generation of HBV gene in transgenic mice.Methods:The analysis of HBV DNA in serum (1～8 months) and integrated HBV DNA in the transgenic mice of 48 #\,87 #\,90 #lines by using nest\|PCR\,Southern blot\,immunohistochemistry.Result:Serum of three lines HBV transgenic mouse is with HBV DNA,and the positive rate in one month of age is the hightest,but the positive rate changes with months,and there are differences between lines\,bodys and months.Conclusion:HBV genes in the transgenic mice may be replicated and have been inherite five generations now.

Key words: HBV transgenic mice, Replication, Generation

摘要： 目的 :研究乙型肝炎病毒 (HBV)转基因小鼠外源基因的复制、传代稳定性。方法 :通过nest PCR和Southern分子杂交等检测方法 ,对 48#、87#、90 #三个系列中各 15只整合阳性小鼠定期进行血清中HBVDNA存在情况以及对各系列繁育F1 F5 代小鼠外源基因整合传代进行检测。结果 :各系列转基因鼠血清都有HBVDNA存在 ,并以 1月龄时血清中HBVDNA阳性率最高 ,但随月龄改变 ,其DNA阳性率也变化 ,并存在系列、个体及月龄上的差异。结论 :HBV基因可在转基因小鼠体内复制、传代 ,且目前已稳定传至第五代 (F5 )。

关键词: HBV转基因鼠, 复制, 传代, 稳定性