Abstract: Objective:To determine the neuronal degeneration in the inner nuclear layer of RCS rat.Methods:The retinal tissue were collected from RCS rats with ages ranged from 18,20,28,35,42,45,56,60,70 to 100 days.The retinas of normal age\|matched SD rats served as control.Immunohistochemical method was applied to label horizontal cell,rod bipolar cells and amacrine cell by using antiserum of calbindin,PKCα and calretinin,respectively.Morphometric study was also performed.Results:Comparing with normal SD rat retinas,retinal degeneration,including outer and inner nuclear layers,was observed in RCS rat retina after postnatal 18-20 day.Morphometric study showed that there was loss of horizontal cells after postnatal 60 days,loss of rod bipolar cells after 20 days,and loss of amacrine cells after 20-35 day in the inner nuclear layer of RCS rats.Conclusions:Our results indicated that there is neuronal degeneration in the inner retina of RCS rat.Transneuronal degeneration may be the mechanism.

Key words: RCS rat, Inner nuclear layer, Transneuronal degeneration

摘要： 目的 :证实先天性视网膜变性 (RCS)大鼠视网膜内核层存在神经原变性。方法 :使用出生后第 18,2 0 ,2 8,35 ,42 ,45 ,5 6 ,6 0 ,70和 10 0d的RCS大鼠视网膜和正常SD大鼠比较 ,利用视网膜内核层细胞标记物 :抗Calbindin ,PKC和Calretinin血清 ,经免疫组化方法分别标记水平细胞、杆双极细胞及无长突细胞 ,并统计这三种细胞的数目。结果 :和正常SD大鼠视网膜比较 ,RCS大鼠从 18～ 2 0d开始视网膜变性 ,除了外核层细胞死亡 ,内核层细胞也有不同程度的丢失 ,并表现为浓染或水肿。统计结果表明 ,内核层水平细胞数目从出生后 6 0d开始减少 ,杆双极细胞在出生后2 0d即数目已减少 ,但可保持至 10 0d才有进一步减少 ,无长突细胞虽然在出生后 2 0～ 35d数目明显减少 ,但可一直保持这个水平。结论 :RCS大鼠视网膜内核层细胞存在神经原变性 ,水平细胞、杆双极细胞、无长突细胞发生损失的时间不同 ,反映了中继神经原变性很可能是这些细胞变性的机理

关键词: RCS大鼠, 视网膜内核层细胞