Abstract: To dynamically observe the levels of inflammatory cytokines in the serum of type 2
diabetes ( T2DM) model in miniature pigs, so as to provide reference data for the study of type 2
diabetes. Method Ten Bama minipigs were randomly divided into a Control group and a model group
(DM) . The Control group was fed with conventional diet, and the DM model in miniature pigs was
established by high-fat diet combinated with intravenous streptozotocin ( STZ) . The experiment lasted for
nine months, fasting blood glucose and insulin were measured monthly, and glucose tolerance was
measured every 3 months. Concentrations of 11 inflammatory cytokines in serum of animals at the 0th,
3rd, 6th, and 9th months were determined by ELISA kit. Result The fasting blood glucose was
significantly increased (P<0. 05) , severely impaired of glucose tolerance and insulin resistance occurred
in the DM group after high-fat diet for 3 months injected with STZ. The ELISA result showed that there was no significant change in the levels of 11 inflammatory cytokines of the DM group at the first 3 months,
and no significant difference compared to the Control group. The levels of platelet-derived growth factorBB ( PDGF-BB) and tumor necrosis factor-α ( TNF-α) were increased after the onset of T2DM and
significantly higher than those of in Control group ( PDGF-BB, P<0. 05 at the 6th month; PDGF-BB and
TNF-α, P<0. 05 at the 9th month) . The level of interleukin-6 ( IL-6) in the DM group was increased
gradually over time which the level at the 9th month was significantly higher than that at the 0 th and 3 r

Key words: type 2 diabetes mellitus, inflammatory cytokines, high-fat diet, STZ, mini-pig

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