Abstract: Objective To investigate researchers ’ cognition levels and their perception of laboratory animal welfare ethics, providing theoretical basis for improving ethical review mechanisms. Methods A retrospective study was conducted by systematically analyzing 412 “ Laboratory Animal Welfare Ethics Review Application Forms” submitted by applicants to the National Natural Science Foundation from Guangdong Medical University in 2020. The content analysis method was employed to evaluate applicants’ comprehension of the 3R principles ( Replacement, Reduction, Refinement ) and their implementation of welfare technical specifications. Results The study revealed that 60% of applications lacked sufficient justification for the number of experimental animals used, while 32. 2% of projects provided incomplete descriptions of pain management protocols. Notably, only 39. 3% of applicants could accurately elaborate on the selection of humane endpoints in experiments, indicating a systematic cognitive bias in animal welfare ethics standards among researchers. Conclusion It is recommended to establish a more comprehensive ethical review system that emphasizes regular training mechanisms for laboratory animal welfare ethics. The 3R principles should be integrated into researchers’ competency evaluation metrics to promote ethical practices through scientific review, thereby effectively ensuring laboratory animal welfare. 

Key words: laboratory animal, ethical review, researchers, knowledge, analysis 

摘要： 目的 探讨科研人员对实验动物福利伦理的认知水平及其影响因素,为完善伦理审查机制提供理论依据。 方法 采用回顾性研究方法,系统分析 2020 年度广东医科大学国家自然科学基金申报者提交的 412 份《 实验动物 福利伦理审查申请表》 ,通过内容分析法评估申请者对实验动物 3R 原则( 替代、减少、优化) 的理解程度及福利技术规范的应用情况。 结果 研究发现 60%的申请材料存在实验动物使用数量论证不足,32. 2%的项目对疼痛管理方案描述不完整。 特别值得注意的是,仅 39. 3%的申请者能准确阐述实验中仁慈终点的选择,反映出科研群体对动物福利伦理标准存在系统性认知偏差。 结论 建议建立更加完善的伦理审查体系,重点加强实验动物福利伦理的常态化培训机制,将 3R 原则认知水平纳入科研人员能力评价指标,以科学审查促进伦理实践,切实保障实验动物福利。 

关键词: 实验动物, 伦理审查, 科研人员, 认知, 情况分析