关键词: 实验动物, 饲料, 垫料, 代谢组学, 标准化

Abstract: Feed and bedding, as key factors in experimental animal research, have a significant impact on the experimental results, but their standardization has not been paid enough attention for a long time. In this paper, we systematically reviewed the mechanisms of feed texture, processing technology and sterilization methods on nutritional metabolism, intestinal microbiota and physiological indicators of experimental animals, and revealed the reproducibility effected by the type and volume of bedding through thermal regulation, behavioral stress and microbial interference. Based on this, this paper proposes that researchers should consider the influence of these factors when conducting relevant experiments to eliminate controllable variability, and suggests the establishment of a feed and bedding standardization system covering the bioavailability of nutrients and processing parameters, and encourages researchers to disclose relevant parameters in research reports to improve the scientific and reproducibility of related studies.

Key words: laboratory animal, feed, bedding, metabolomics, standardization