Objective Verify the objectivity and accuracy of the “—” Maze in testing animal anxiety states, actively stimulate exploratory behavior in rats and mice, and effectively solve the testing defects of the elevated plus maze (EPM) in the anxiety triple testing device ( open field+elevated plus maze+ light- dark box) . Method ①Conduct a “—” elevated maze test and compare it with EPM; ②Through open field test and light-dark boxtest, detect whether the “—” elevated maze has other behavioral effects on animals; ③Verify the testing effect of “—” Maze through drug interventions ( such as Diazepam, DZP, the anxiety therapy drugs) . Result Compared with EPM, the “—” Maze group showed a significant increase in open arm entry time and frequency ( P<0. 05, P<0. 001) . Compared with the EPM group, the EPM + Diazepam group ( EPM + DZP ) showed a significant increase in open arm entry time and frequency ( P < 0. 05, P < 0. 01 ) . Compared with the EPM group, the EPM + DZP group showed a significant increase in the distance and number in the central area of the open field test ( P < 0. 05) . Compared with the “—” Maze group, the “—” Maze +Diazepam group ( “—” Maze +DZP ) showed a significant increase of the distance, entry time, and frequency in the central area of the open field test (P<0. 05) . Compared with the EPM group, the EPM+DZP group showed a significant increase in the time and frequency of light-dark box test ( P<0. 05, P<0. 01) . Compared with the “—” Maze group, the “—” Maze+DZP group showed a significant increase in the time and frequency of light-dark box test (P< 0. 05) . Conclusion The open arm entry time and frequency of the “—” Maze are significantly better than those of EPM, and do not have any other effects on animal behavior. It is more suitable for anxiety model testing and is worth further research and development and promotion.