关键词: <, p>, 禽白血病病毒, 液相芯片, 单克隆抗体, 特异性<, /p>

Abstract: Abstract: Objective To establish the liquid phase chipmethod of rapidly detecting avian leucosis virus，and provide technical basis for the detection of large quantities of samples． Method Prepare matching monoclonal antibody against P27 protein of avian leucosis virus，biotin labelling antibody，magnetic beads coupling capture antibody． this method is validated with Luminex200 analysis system to test specificity，sensitivity and repeatability．Ｒesult The positive criterion is more than twice as much as the blank control． The specificity test result showed that there was no cross-reaction between many other pathogens of the chicken，meaning good specificity． Simultaneously repetitive experiment shows the coefficient of variation is within 7% ，which indicates that the stability of the experiment is good and repeatable． Conclusion The Luminex liquid chip technology，which directly detects the ALV pathogen，is specific，stable and repeatable．

Key words: <p>Avian leucosis virus, the liquid phase chips, monoclonal antibodies, specificity</p>