关键词: 阿尔茨海默病, β-淀粉样蛋白, 焦虑, 抑郁, 涤痰汤

Abstract: Objective　To observe the behavioral characteristics of male 5×FAD mice and intervention effect of Ditan Tang.Methods　Positive mice were screened through tail gene identification, and their learning and memory abilities were evaluated by Barnes maze test(BMT) and novel object recognition (NOR) test. The anxiety status of mice was evaluated by open field test (OFT) and elevated plus maze (EPM) test. The depression status of mice was evaluated by sucrose preference test(SPT), tail suspension test (TST), and forced swimming test (FST). The social ability of mice was evaluated by three-chamber social preference test(TCSP). Observe the intervention effect of Ditan Tang on the behavior of mice of different ages.Results　There was no significant difference in the general condition between 5×FAD mice and C57/BL6 mice. Compared with control group, in BMT, 4, 8, and 12 months’ old 5 × FAD mice showed varying degrees of reduction in the number of correct hole explorations (P<0.05, P<0.01) and in the correct quadrant dwell time (P<0.01). In NOR test, the cognitive coefficient of 4, 8, and 12 months’ old 5×FAD mice decreased to varying degrees (P<0.05).In OFT, the total activity distance, central area dwell time, and upright exploration frequency of 4, 8, and 12 months’ old 5×FAD mice were reduced to varying degrees (P<0.01). In EMP test, the open arm dwell time and downward exploration frequency of 4, 8, and 12 months’ old 5×FAD mice decreased to varying degrees (P<0.01), while the closed arm dwell time increased to varying degrees (P<0.01). In TST and FST, the immobility time of 4, 8, and 12 months’ old 5×FAD mice increased to varying degrees (P<0.01). In SPT, the sucrose skewness of 4, 8, and 12 months’ old 5×FAD mice was reduced to varying degrees (P<0.01). Ditan Tang significantly improved the above indicators in 4-month-old 5 × FAD mice, and partially improve some indicators in 8- and 12-month old 5×FAD mice.Conclusion Ditan Tang can effectively improve the anxiety and depression status of 4-month-old male 5×FAD mice.It provides theoretical basis for clinical application.

Key words: Alzheimer disease, β-amyloid protein, anxiety, depression, Ditan Tang