关键词: 小鼠模型, 结直肠癌肝转移, 肠系膜上静脉注射

Abstract: Objective　To establish a mouse model of colorectal cancer liver metastasis (CRLM) by using a modified superior mesenteric vein injection technique, aiming to optimize modeling efficiency and stability, thereby providing a reliable animal model for in vivo experimental research on CRLM.Methods Forty male BALB/c mice were selected. The distal superior mesenteric vein was ligated, and 100 μL of a CT26-luc colorectal adenocarcinoma cell suspension (1×106 cells/mL) was injected to construct the CRLM model. Model establishment was verified using small animal in vivo fluorescence imaging and hematoxylin-eosin (HE) staining.Results　In vivo fluorescence imaging revealed punctate fluorescent signals in the livers of some mice as early as day 2 post-modeling. Stable tumor formation was generally achieved within 4-6 d, with a modeling success rate of 95% and a mortality rate of 5%. Gross anatomical examination showed multiple, scattered grayish-white metastatic foci on the liver surface. HE staining confirmed the presence of multiple, dispersed nuclear atypia lesions within liver tissue. Conclusion　The modified method for establishing mouse CRLM model is highly stable and reproducible, and can serve as a high-quality animal model to support fundamental research on the diagnosis and comprehensive treatment of CRLM.

Key words: mouse model, colorectal cancer liver metastasis, superior mesenteric vein injection