急性呼吸窘迫综合征, 动物模型, 模型优化

Objective To improve the success rate of ARDS disease model, we explored the optimal condition for constructing the model through endotracheal instillation of LPS, eventually providing a technical support for the basic research of ARDS. Methods A total of 116 C57 / BL6N mice were included in this research.We explored the optimal administration conditions by comparing the drug distribution and actual modeling effects of different body positions ( slant position, supine position) and LPS volumes ( 30 μL / 20 g, 60 μL / 20 g, 90 μL / 20 g ) . Further, different anesthesia methods were compared according to the physiopathological status of mice. The above optimized conditions were validated by ARDS modeling. Results Compared to the supine position, LPS instilling to mice in a slant position can distribute more evenly across lung lobes. A dosage of 60 μL LPS / 20 g body weight can meet the modeling requirement, such as thickening of alveolar walls and increasing of neutrophils according to  H&E staining. Compared to the control group, significant differences were observed in injury score,vascular permeability and systemic inflammation(P<0. 001) . Last but not least, intraperitoneal injection of anesthesia exacerbates inflammation in mice and affects blood oxygen saturation ( P < 0. 01 ) .Conclusion C57 / BL6N male mice (6-8 weeks old, 20 g body weight) were used for establishing an ARDS disease model. Isoflurane inhalation anesthesia was selected before surgery, which was performed in a slant position. The optimal volume of LPS was 60 μL / 20 g ( body weight) for inducing ARDS. Tribromoethanol by intraperitoneal injection was not recommended for anesthesia, due to side effects such as extra inflammatory burden and reduction of blood oxygen saturation.

:acute respiratory distress syndrome, animal model, model optimization