关键词: 脱细胞生物敷料, 小型猪, 烧烫伤, 创面愈合

Abstract: Objective To study the effect of acellular dermis matrix on pigs with deep partial thickness burn. Method Twenty-four male and female miniature pigs, weighing ( 15 ± 3 ) kg, were included. They were used to prepare the deep partial thickness burn model with the size of 4 cmX6cm by copper ingots, then randomly divided them into two groups: experimental group using a cellular dermis matrix and blank control group using absorbent cotton gauze. Detected the rate of wound healing, healing time, clinical and histopathological observation, hydroxyproline contents of wound in postoperative 1,2,3 and 4 weeks. Result During the experiment, all the animals survived , and no wound infection occurred. The wound surface of the experimental group was dried after operation, 2 weeks incrustation, 3 weeks decrustation and hair growth. In the blank control group, local exudation occurred on the wound surface at 1 weeks after operation, and the rest of the wound wasdry, incrustation at 2 weeks, decrustation and covered with hair at 4 weeks. The time of wound healing was ( 20. 83 ± 0.75 ) d in the experimental group and ( 27. 33 ± 0. 82 ) d in the blank control group. There was significant difference in wound healing rate between the two groups at 2 weeks and 3 weeks. The skin of the experimental group had clear structure at 3 weeks after surgery and the burn wound was basically healed, and after 4 weeks basic skin structure was intact. The formation of basic skin structure and append ages in the blank control group lagged by 6 days compared with the experimental group .There were significant differences in inflammatory cell infiltration and hydroxyproline contents of wound between the two groups at 1 weeks, 2 weeks and 3 weeks. Conclusion Acellular dermis matrix could effectively promote the healing of burn wound, and shorten the treatment cycle.

Key words: Acellular dermis matrix biological dressings, miniature pigs, burns, wound healing