关键词: 蓝光, 视网膜衰老, 精神疾病, 应激反应

Abstract: Objective To investigate the effects of red and blue light on retinal senescence and neural network in rats. Method Fifty male Wistar rats were randomly divided into 5 groups : blue light irradiation 1 h、blue light irradiation 8 h、red light irradiation 1 h,red light irradiation 8 h and blank control group. A compulsory swimming experiment ( FSTs) was performed after 12 weeks of intervention to assess mental state. The primary culture of fresh retinal pigment epithelial cells ( RPE) of the eyeball was dissected and senescence-associated-identification of β- galactosidase ( SA-β-Gal ) activity, analysis of the association between illumination time and replicative senescence, and use of reagents box to detect nitric oxide ( NO) and nitric oxide synthase ( NOS) content in brain tissue and analyze the stress response of light stimulation to rats. Result Compared with red light irradiation , blue light irradiation 8 h significantly up-regulated the proliferation of RPE cells (P <0. 05 ) and the content of ( P< 0.01 ) . At the same time, blue light irradiation 8 h more easily caused rats to fall into a nervous and desperate mental state. Conclusion The effect of blue light on retinal senescence and neural network is greater than that of red light.

Key words: Blue light, Retinal aging , Mental illness, Stress response