关键词: 放射, 肺炎, 病理观察

Abstract: Objective To clarify development of the pathological process of radiation pneumonia. Method Using 6MV X-ray, of which the cumulative dose is 20Gy, irradiates the full chest once. The hematoxylin-eosin staining was conducted respectively on the 7th day, 14th day and 28th day after irradiation for pathological study. In addition, the Image-Pro plus analysis system was applied to quantitatively analyze thickness of alveolar walls, and surface mass density of alveolar spaces and interstitial tissue. Result Alveolar atrophy, alveolar septum widening and inflammatory cell infiltration were observed along with the development process. The collapsed or consolidated alveolus and mild pulmonary fibrosis were observed after 28 days. Furthermore, the differences of thickness of alveolar walls and surface mass density of alveolar spaces and interstitial tissue were very significant. Conclusion Radiation pneumonia model in Kunming Mouse could be replicated successfully by the mentioned method, of which the pathological changes are in accordance with the clinical course.

Key words: irradiation, pneumonia, pathological observation