关键词: 动物福利, 束缚, 行为学

Abstract: Objective To study the behavioral and NF-κB expression changes in welfare impaired rats caused by restrain-stress. Method Wrap restraint stress (WRS) were administrated by using tubular container within male SD rats (± 200 g). At time points of 0, 1, 3, 7, 10 and 14 days after WRS administration, open filed test and elevated o-maze test were employed to analyze the physical behavior changes of SD rats. NF-κB p65 mRNA transcription levels and protein expression in all SD rats were examined as well. Result Significant expression enhancements were observed both in NF-κB p65 mRNA transcription and NF-κB p65 protein expression at the 3rd day after WRS administration compared with pre-procedure (P<0.05), but no behavior changes could be found. At the 7th day after WRS administration, significant decline (P<0.05) in physical activity capacity and activity intension were noticed. All SD rats exhibited symptoms of depression and dread. Conclusion Changes in NF-κB mRNA and protein expression may prior to behavioral changes in welfare impaired rats.

Key words: animal welfare, restrain-stress, behavior