关键词: 树鼩, 骨髓间充质干细胞, 成骨细胞, 诱导

Abstract: Ob jective To investigate the effects of different mediums on the differentiation of tree shrew bone marrow mesenchymal stem cells（BM-MSCs） into osteoblasts in vitro.Method Tree shrew bone marrow mesenchymal stem cells（MSCs） were isolated and cultured in vitro by density gradient centrifugation and adherent culture.The tree shrew bonemarrow mesenchymal stem cells of P3 generations were divided into 7 groups.Group A：high glucose DMEM+1 μmol/L dexamethasone+100 μmol/L vitam in C+10 mmol/Lβ-glycerophosphate；Group B：high glucose DMEM+50 ng/m L BMP-2；Group C：high glucose DMEM+80 ng/m L BMP-2；Group D：high glucose DMEM+50μmol/L Vitam in C+10 mmol/Lβ -glycerophosphate+20 ng/m L BMP-2；Group E： high glucose DMEM+1 μmol/L dexamethasone+100 μmol/L Vitamin C+20 mmol/L βphosphoglyceride； Group F： high glucose DMEM+100 μmol/L dexamethasone+100 μmol/L Vitam in C+10 mmol/Lβ-phosphoglyceride；Group G：DMEM/F12+0.1μmol/L dexamethasone+50μmol/L Vitamin C+10 mmol/Lβ-phosphoglyceride.After 18 d，stain the cells with alkaline phosphatase and alizarin red.Result The result of each group of alkaline phosphatase staining were showed positive in different degree；the result of alizarin red staining in group A，B，C，D and group E were observed significantm ineral nodules.Conclusion Group A，group B，group C，group D and group E induced differentiation mediums could induced tree shrew BM-MSCs to differentiate into osteoblasts，among which group A and group B were the most effective.

Key words: Tupaia, Bone marrow mesenchymal stem cells, Osteoblasts, induction