关键词: 分装冻融, SD大鼠, 血清

Abstract: Ob jective To investigate the effect of sub packaged freezing and thawing on serum biochemical test in SD rats at-20℃for cryopreservation.Method A fter the instrument was calibrated and quality control was passed，the blood of 40 blank female SD rats was collected，stored for 30 min at 2～8℃，then centrifuged，the serum was dispensed and sealed with a sealing film，and each animalwas dispensed in 4 portions.Serum detection analysis was done under room temperature for 4 h and the resultwas the reference level.The remaining 3 sera were stored at-20 ℃ for cry preserved and were re-thawed and detected after 2 d，7 d，14 d respectively.The effects of biochemical test result at room temperature for 4 h were compared with those at-20℃for cryopreservation for different time.Result There were statistical differences of the concentration of TP，ALB，GLU and CREA in the serum biochem ical test result between the condition of freezing-thawing at-20℃for 14 d and room temperature for 4 h （P＜0.05）.There were statistical differences of the concentration of ALT， TP， ALB， GLU， UREA，CREA and TC in the serum biochemical test result between the condition of freezing-thawing at-20℃for 7 d and room temperature for 4 h （P＜0.05）.There were statistical differences of the concentration of ALT， TP， ALB，GLU and CREA in the serum biochem ical test result between the condition of freezing-thawing at-20℃for 2 d and room temperature for 4 h（P＜0.05）.There was no statistical difference of the concentration of AST and TG in the serum biochemical test result between the condition of freezing-thawing at-20℃ for 2 d，7 d，14 d and room temperature for 4 h （P＞0.05）.Conclusion The serum biochemical test result of AST and TG were stable and consistent between the condition of freezing and thawing at-20℃ for 2 d，7 d，14 d and room temperature for 4 h while the other seven result have changed.This experiment shows that m inim ize the number of serum freezethawing as few as possible if there is no special circumstance，and suggests that it is best to detect in that day otherwise itwill affect the accuracy of the test result.

Key words: sub packaged freezing-thawing, SD rats, serum