关键词: 长爪沙鼠, Ⅱ型糖尿病, Nf-κb, 实时定量荧光PCR, Western blot

Abstract: Objective To investigate the mRNA and protein expression level of Nf-κb in 6 tissues of diabetic gerbils, and to explore the role and target tissue of Nf-κb in diabetic gerbil model. Method We selected 6 animals from diabetic and control group, respectively; and collected skeletal muscle, liver, adipose, kidney, heart and brain from each group. The expressionsat mRNA and protein level of Nf-κb were evaluated by Q-PCR and Western blotting in diabetic and control groups. Result Compared with control group, mRNA expression of Nf-κb had an increasing trend in skeletal muscles, kidney and brain of diabetic gerbil. The mRNA level of Nf-κb significantly decreased in adipose and showed decreasing tendency in liver and heart. After detecting protein expression, we found the protein level was consistent with mRNA in all tissues except liver. Conclusion Adipose tissue is the target tissue of Nf-κb in diabetic gerbil.

Key words: Mongolian gerbils, Type 2 diabetes, Nf-κb, real-time PCR, Western blot