关键词: 甘油-3-磷酸, 甲肝疫苗, 铝佐剂, 体液免疫

Abstract: Objective To investigate the effect of G3P(Glycerol-3-phosphate) on humoral immune response induced by hepatitis a virus vaccine in mice. Method Forty nine ICR female mice were divided randomly into 7 groups (7 mice in each group): negative control group, antigen group, aluminum adjuvant group and 4 groups of G3P. The specific anti-HAV IgG levels in the serum were detected by enzyme-linked immunosorbent assay (ELISA) at the 4th, 8th, 12th, 16th, and 20th week after immunization. Result The serum anti-HAV IgG levels were detected in antigen group, aluminum adjuvant group and 4 groups of G3P at the 4th, 8th, 12th, 16th, and 20th week after immunization, and the serum IgG level of the mice increased first and then decreased, and the level reached the highest at 8th week. The serum IgG level of G3P group was higher than the antigen group and the difference was statistically significant (P<0.05)at 4th week and 8th week, besides, the serum IgG level of G3P group was higher than aluminum adjuvant group at 4th week and the difference was statistically significant(P<0.05).The serum IgG level of G3P 2 mg was the highest in all of G3P,and the level was higher than the antigen group at 4th, 8th, 12th, 16th, and 20th week after immunization and reached the peak at the 8th week, with the value of lg(3.064 ± 0.07851), aluminum adjuvant also reached the peak at 8th weeks, with the value of lg (3.150 ± 0.1382). Conclusion G3P could significantly and rapidly enhance humoral immune response induced by HAV vaccine in mice; the result suggest it may be used to develop a new type of human vaccine adjuvant．

Key words: G3P, Hepatitis a virus vaccine, Aluminum adjuvant, humoral immunity