实验动物科学 ›› 2016, Vol. 33 ›› Issue (02): 6-13.

• 研究 论著 • 上一篇    下一篇

实验红鲫对137Cs 辐射的氧化应激响应

  

  1. ( 1． 南华大学药学与生物科学学院，衡阳421001) ( 2． 湖南省永州职业技术学院，零陵425100)( 3． 湖南交通工程学院，衡阳421001)
  • 出版日期:2016-04-30 发布日期:2017-05-31
  • 基金资助:

    <p>基金项目: 国家科技支撑计划项目( No． 2015BAI09B05 ) ; 湖南省重点研发计划项目( No． 2015DK3004 ) ; 湖南省科技计划重点项目( No． 2014TT1002)</p>

Oxidative Stress Ｒesponse to 137Cs Ｒadiation in Ｒed Crucian Carps

  • Online:2016-04-30 Published:2017-05-31

摘要： 摘要: 目的探讨实验红鲫对137 Cs 辐射的氧化应激响应和氧化应激生物标记物。方法实验红鲫分别以0 Gy、1. 94 Gy、3. 88 Gy、7. 76 Gy、15. 53 Gy137Cs 辐照，分别于24 h，48 h，72 h，96 h 取出肝脏、性腺，按试剂盒方法检测SOD、GSH-PX 活性，于7 d 和14 d 取出肝脏、肾脏、心脏、脑，按Western blot 方法检测HSP70 含量。结果1. 94 Gy至15. 53 Gy137Cs 辐照能引起实验红鲫氧化应激，SOD、GSH-PX 活性和HSP70 含量发生改变。1. 94 Gy137Cs 辐照诱导实验红鲫肝脏SOD 活性升高， 3. 88 Gy、7. 76 Gy、15. 53 Gy137Cs 辐照诱导实验红鲫肝脏SOD 活性下降，且随辐射剂量升高，其SOD 活性逐渐降低。1. 94 Gy 以上137Cs 辐照诱导实验红鲫性腺SOD 活性下降，并随辐射剂量升高和时间的延长而下降，且下降幅度较大。各辐照处理组实验红鲫肝脏和性腺的GSH-PX 活性变化不大，表现出随辐射剂量升高则活性降低的变化趋势。137Cs 辐照处理后7 d 和14 d，各辐照处理组实验红鲫肝脏、肾脏、心脏和脑的HSP70 表达量均比对照组的高，且随着137Cs 辐照剂量的增加和时间的延长而升高，均存在着一定的“剂量－ 时间－效应”关系。结论实验红鲫性腺对137Cs 辐射的氧化应激响应较敏感，性腺SOD 和肝脏或肾脏的HSP70 可作为氧化应激标志物。

关键词: <, p>, 实验红鲫, 137Cs, SOD, GSH-PX, HSP70<, /p>

Abstract: Abstract: Objective To study the oxidative stress response and oxidative stress biomarkers in red crucian carp following 137Cs radiation． Method The red crucian carps were irradiated with 0 Gy，1. 94 Gy，3. 88 Gy，7. 76 Gy，15. 53 Gy 137Cs，respectively，and the liver and gonad were collected 24 h，48 h，72 h，96 h after irradiation，respectively． SOD and GSH-PX activity were detected by commercially available kit; the liver，kidney，heart，brain were collected 7d and 14d after irradiation，respectively，and HSP70 content was detected by western blot． Ｒesult 1. 94 Gy to 15. 53 Gy137 Cs irradiation could induce the oxidative stress and could change SOD and GSH-PX activity，and HSP70 content in red crucian carp． 1. 94 Gy137Cs irradiation induced hepatic SOD activity increased，the SOD activity increased with the prolongation of observation time． 3. 88 Gy，7. 76 Gy，15. 53 Gy137Cs irradiation induced hepatic SOD activity decreased，and the SOD activity decreased gradually with the increase of 137Cs radiation dose． 137Cs irradiation at more than 1. 94 Gy doses induced gonadal SOD activity decreased，and gonadal SOD activity gradually decreased significantly with the increase of 137Cs radiation dose and with the prolongation of observation time． The GSH-PX activity in the red crucian carp liver and gonad showed little change in each 137Cs exposed group，which showed the trend of decrease with the increase of radiation dose． At 7 d and 14 d after 137Cs irradiation，the HSP70 expressions in liver，kidney，heart and brain of red crucian carps in each 137Cs exposed group were higher than that in control group． The HSP70 expression increased with the increase of 137Cs radiation dose and with the prolongation of observation time． There was some " dose-time-effect”relationship．Conclusion The gonad of red crucian carps was more sensitive to the oxidative stress response of 137Cs radiation，and the SOD in gonad and HSP70 in liver or kidney could be used as oxidative stress biomarkers．

Key words: <p>red crucian carp, 137Cs, SOD, GSH-PX, HSP70<p>

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