关键词: 间充质干细胞, 诱导分化, 肝样细胞

Abstract: Abstract: The best treatment of severe liver disease caused by a variety of factors have been proved to be orthotopic liver transplantation，but the lack of liver donors and a series of problems caused by immune rejection greatly limits the use of the procedure，while in the screening of liver-related drugs ，primary hepatocyte cell is difficult to culture and easy to mutate in the training process． And at the same times ，with the depth of bone marrow mesenchymal stem cell research，more and more evidence indicate that bone marrow mesenchymal stem cells have potential to differentiate into hepatocyte-like cells． Thus，hepatocyte-like cell differentiated from bone marrow mesenchymal stem cells has good prospects in the field of regenerative medicine and drug screening． In this paper ，we summarize the isolation and biological characteristics of mesenchymal stem cells，inducing culture conditions，biological characteristics and clinical prospects of hepatocyte-like cells．

Key words: mesenchymal stem cell, differentiation, hepatocyte-like cell