关键词: 大黄牡丹汤组方, 急性胰腺炎, 炎症介质, 氧自由基

Abstract: Abstract: Objective To study the effect of Dahuang Mudan Decoction ( RPDP) on inflammatory response and oxidative stress in acute pancreatitis( AP) model rats． Method Divided 90 SD rats randomly into three groups: the sham operation( SO) group ，the AP group，and the RPDP group． The models of AP were made by pressurize inject 3% sodium taurocholate into the bile duct where was 0.3mm away from the pancreatic duct with the speed of 0. 1ml /min． The rats in the RPDP group were lavaged with RPDP ( 40 g /kg) after made AP model． The rats in the SO group and the AP group were lavaged with partes aequales Sodium Chloride． Sacrifice the rats 4 h，8 h，12 h hours after establish the animal model，separated the serum and detected the content of IL-6，IL-β and TNF-α in the serum，cut pancreatic tissue in every time quantum and detected the content of MDA，MPO and SOD． Result Compared with the AP group，the serum content of IL-6，IL-β and TNF-α were significantly decrease in RPDP group( P ＜ 0. 05) ，the content of MDA and MPO in pancreatic tissue in every time quantum were also decrease in RPDP group( P ＜ 0. 05) ，while the content of SOD were significantly improve( P ＜ 0. 05) ． Conclusion Dahuang Mudan Decoction could repress the release of TNF-α，IL-6 and IL-β，could decrease the product of MDA，and had the effect of anti-oxidative damage，cleaning oxygen free radicals．

Key words: Dahuang Mudan Decoction, acute pancreatitis( AP) , mediators of inflammation, oxygen free radical