关键词: DNA 提取, 基因组 DNA, 血凝块, PCR

Abstract: Abstract: Objective To compare the differences of three methods for DNA extraction from guinea pigs blood genomic DNA． Method Fresh anticoagulant NaI manual method，Blood clot manual method and DNA extraction kit method were used to extract blood genomic DNA from 20 guinea pigs． The integrity ，concentration ，purity and the effects of PCR amplification of obtained DNA were compared by Spectrophotometer，agarose gel electrophoresis， PCR amplification and other methods． Result The highest DNA integrity，concentration and purity could be achieved by employing fresh anticoagulant NaI manual method． The DNA concentration of blood clot manual method was approximate with the DNA concentration of fresh anticoagulant NaI manual method，but the purity was the lowest，and have some degree of breakage and degration． The DNA concentration obtained by DNA extraction kit was the lowest，integrity and purity were in the middle． There was no differences in PCR amplification results of the three methods． Conclusion The genomic DNA could be extracted by the three methods and could meet the requirements of subsequent PCR amplication，but each of them has its advantages and disadvantages，researchers could choose the suitable method according to specific conditions．

Key words: DNA extraction, genomic DNA, blood clot, PCR