关键词: 猫疱疹病毒 1 型, ELISA, 猫

Abstract: Abstract: Objective To establish a ELISA method for detection of Felid herpesvirus 1( FHV-1) antibody in cat． Method Raises the CRFK cell，vaccinate the FHV-1 virus，prepare the normal CRFK antigen and special FHV- 1 antigen，titrate the best working density of enzyme union and the normal or special antigen and carry out the experiments of accuracy，sensitivity，stability and specificity． Result The best working density of normal，special antigen and the enzyme union is 0. 05 μg /mL，10 μg /mL and 1∶ 5 000 respectively; The inter-assay coefficient of variation of normal antigen and special antigen is 8. 7% and 5. 8% respectively，the intra-assay average coefficient of variation is 11. 9% and 6. 6% respectively; The detection sensitivity is 1∶ 6 400． There is no cross-reactivity with Feline panleukopenia virus ( FPV) and Feline calicivirus virus ( FCV) ． The stability test shows the relative deviation is below 25% ． Conclusion The ELISA method is good in duplication，stability，specificity，and sensitivity，as a result of which ELISA can be used in detecting the Cat FHV-1 immune body．

Key words: FHV-1, ELISA, cat