关键词: 啮齿类实验动物, 病理学诊断, 脏器病变

Abstract: Abstract: Objective In May 2014，our lab has taken samples from 14 units to investigate the health status of Beijing laboratory rodents． Method We collected the heart，liver，spleen，lung，kidney，large intestine and small intestine，and put these organs into 10% paraformaldehyde solution for fixation，and then prepared into two different sections for optical microscopy observation including all paraffin specimens stained with H． E． and the frozen sections stained with Oil Red O and PAS． Result The vast majority of laboratory rodents were pathologically diagnosed as healthy animals． Liver cell swelling( 32. 6% ) ，slight lung lesion( 24% ) and protein cast of kidney( 13. 2% ) were main lesions which might affect the health of laboratory rodents． Comparing with the results of the last two years，Oil Red O staining positive rate( 6. 2% ) ，which was lower than 17. 1% in May 2013， 6. 25% in October 2013 ，26. 7% in May 2012 and 14. 8% in October 2013 had a tendency to reduce At the beginning ，kidney lesion was only found occasionally in mice ( 2% ) in 2012 and rats ( 20% ) in 2011，but became common in mice( 6% ) ，rats( 12% ) and guinea pigs( 20% ) ． Kidney lesion． changes from accidental occurrence to frequent occurrence，showed that prevention should be strengthened． Conclusion The vast majority of laboratory rodents were pathologically diagnosed as healthy animals． The result might be related to breeding and feeding environment and transport stress，and the improvement of management level and avoidance of stress can ameliorate the health status of laboratory rodents．

Key words: laboratory rodents, pathological diagnosis, organ diseases