关键词: 大鼠, 血常规, 采血方法

Abstract: Abstract:Objective To compare the hematological indexes of blood samples collected from different ways in Wistar rats. Method Blood samples were collected from tail, orbital and abdominal aorta of 40 SPF Wistar rats and were detected by Nihon Kohden MEK - 6318K hematology analyzer. The parameters of WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, LYM, MID and GRN from three sampling ways were analyzed and pairwise compared using paired t test. Result WBC count in the tail blood was the most, and following was the orbital blood, and WBC count in the abdominal aorta blood was the least, which was 1/3 of that in the tail blood. There was highly significant difference between the three methods (P 0.05). However in female rats, HGB and RBC count in the tail blood were higher than those in the orbital blood (P<0.01). PLT count in the peripheral blood (the tail blood and the orbital blood) was less than that in the abdominal aorta blood with highly significant difference ( P< 0.01). Conclusion There were significant differences between the three methods, so each laboratory should set up own basic data for blood routine.

Key words: rat, blood routine, blood sampling method