关键词: 动脉粥样硬化, 斑块形成, 脂质水平, 炎症因子, 血小板聚集, 血管活性物质

Abstract: Abstract: Objective To investigate the relative factors for experimental atherosclerotic plaque of rabbit induced by immunolesion and high fat diet． Method 30 Japanese white rabbits，intravenously injected bovine serum albumin ( 250mg / kg) ，feeding high fat diet after injection． 7 days later，injected again bovine serum albumin ( 250mg / kg) ． Meanwhile，established purely high fat diet group and normal diet group as control，10 rabbits each group． 72 days later，got blood from central artery of rabbit ears to determine lipid level，inflammation factors，vasoactive substance，platelet aggregation and vascular endothelium，etc． and analyzed which related to the pathological change of atherosclerotic plaque or not with SAS statistic software． Result After 72 days，the aorta of both the test group and the purely high fat diet group formed plaque． The plaque of the test group is localized，which is similar to the human being． The lipid level and the content of inflammatory factors，e． g． ，interleukin － 6 ( IL － 6 ) ， interleukin － 8 ( IL － 8 ) and high sensitivity-C reactive protein ( hs-CRP) are obviously higher than the control group; the content of vasoactive substance and thromboxane B2( TXB2) changed significantly than the control group ( P ＜ 0. 05) ． By the Pearson correlation coefficient statistics processing，And above factor into positive correlation to the plaque size induced by immunolesion and high fat diet; high density lipoprotein ( HDL-C，r = － 0. 58 ) ) ， nitric oxide ( NO，r = － 0. 26) are negatively related with the plaque size，and the correlation coefficient of HDL-C is the greatest． Conclusion The rabbit atherosclerotic plaque induced by immunolesion and high fat diet formed in a short time with obvious pathological change，which is similar to that of human beings and closely related to lipid level，inflammation factor，platlet aggregation and endothelial factor，which further show it more reasonable for evaluation the atherosclerosis．

Key words: atherosclerosis, plaque, lipid level, inflammation factors, platlet aggregation, vasoactive substance