微卫星标记预警实验红鲫对苯酚遗传毒性响应的研究

实验动物科学 ›› 2013, Vol. 30 ›› Issue (02): 23-31.

微卫星标记预警实验红鲫对苯酚遗传毒性响应的研究

( 南华大学药学与生物科学学院实验动物学部，衡阳421001)

出版日期:2013-06-29 发布日期:2014-05-12
基金资助:
湖南省自然科学基金项目( No． 05JJ30046) ; 湖南省科研条件创新专项( No． 2010TT1007) 基金资助

Microsatellite Marker of Early Warning Experiment Research on Genotoxicity of Phenol in Red Crucian Carp C1HD

Online:2013-06-29 Published:2014-05-12

摘要/Abstract

摘要： 摘要: 目的探讨微卫星标记预警实验红鲫对苯酚遗传毒性的响应。方法选择5 个微卫星标记，观察4 个苯酚染毒组经8 d 染毒实验，苯酚对实验红鲫C1HD 系微卫星多态性的影响。结果苯酚在实验红鲫C1HD 系体内的富集提高了基因杂合度( H) 、基因多态性信息含量( PIC) 等群体遗传学指数。在1 /8 LC50苯酚浓度组染毒第6 天，基因杂合度( H = 0. 15 ± 0. 19) 和基因多态性信息含量( PIC = 0. 42) 均达到峰值。结论体内富集一定浓度的苯酚可致实验红鲫C1HD 系微卫星多态性改变。微卫星基因杂合度( H) 和多态性信息含量( PIC) 随苯酚染毒时间的延长和富集量的增加而升高。

关键词: 微卫星标记, 苯酚, 实验红鲫C1HD 系

Abstract: Abstract: Objective To study the microsatellite markers of early warning on the response of genotoxicity of phenol in red crucian carp C1HD． Method The red crucian carp C1HD were divided into 4 exposure groups and 1 control group and choose 5 microsatellite markers to study the effect of phenol on the microsatellite polymorphism after exposed to different concentration phenol for 8 d． Result The indexes were increased by the enrichment of phenol in red crucian carp C1HD，such as the gene heterozygosity ( H) ，gene polymorphism content ( PIC) ，and so on． In the 8th day，the gene heterozygosity ( H = 0. 13 ± 0. 19) reached the peak value after exposed to thephenol of 1 /2 LC50 And in the 6th day，the gene polymorphism content( PIC = 0. 42) reached the peak value after exposed to the phenol of 1 /8 LC50 ． Conclusion The microsatellite polymorphism will be changed in the red crucian carp C1HD after phenol was enriched to a certain concentration． The microsatellite heterozygosity ( H) and polymorphism information content ( PIC) will be increased with the enrichment of phenol and the extension of exposure time．

Key words: microsatellite markers, phenol, red crucian carp C1HD

中图分类号:

Q95 － 33

朱晓娟, 董先辉, 练高建, 吴端生. 微卫星标记预警实验红鲫对苯酚遗传毒性响应的研究[J]. 实验动物科学, 2013, 30(02): 23-31.

ZHU Xiao-Juan, DONG Xian-Hui, LIAN Gao-Jian, WU Duan-Sheng. Microsatellite Marker of Early Warning Experiment Research on Genotoxicity of Phenol in Red Crucian Carp C1HD[J]. laboratory animal science, 2013, 30(02): 23-31.