Table of Content

28 December 2024, Volume 41 Issue 6

Previous Issue   

Organoids are Expected to be one of the Ethical Problems of Experimental Animals

REN Meidan, ZHU Jinqiang

2024, 41(6):  1-4.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 001

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The ethics of experimental animals has been a issue that cannot be ignored in animal experiments and attracts increasing attention. With the implementation of the “ 3R” ( replacement, reduction, refinement) principle of experimental animals and various laws and regulations guaranteeing the welfare of experimental animals, it is imperative to develop the replacement technology of experimental animals. In recent years, various organoid technologies have developed rapidly and shown great advantages, which is expected to be one of the ways to solve the ethical problems of experimental animals. This paper summarizes the problems in animal experiments and the development and application of organoids, in order to provide new ideas for the development of replacement technology of experimental animals and solve the ethical problems of experimental animals.

Application of “—” Maze in Behavioral Testing of Mice

GAO Mingzhou, HU Minghui, ZHOU Wanqing, LIU Mengdi, HAN Muxuan, GENG Xiwen, WEI Sheng, ZHANG Hao

2024, 41(6):  5-10.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 002

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Objective Verify the objectivity and accuracy of the “—” Maze in testing animal anxiety states, actively stimulate exploratory behavior in rats and mice, and effectively solve the testing defects of the elevated plus maze (EPM) in the anxiety triple testing device ( open field+elevated plus maze+ light- dark box) . Method ①Conduct a “—” elevated maze test and compare it with EPM; ②Through open field test and light-dark boxtest, detect whether the “—” elevated maze has other behavioral effects on animals; ③Verify the testing effect of “—” Maze through drug interventions ( such as Diazepam, DZP, the anxiety therapy drugs) . Result Compared with EPM, the “—” Maze group showed a significant increase in open arm entry time and frequency ( P<0. 05, P<0. 001) . Compared with the EPM group, the EPM + Diazepam group ( EPM + DZP ) showed a significant increase in open arm entry time and frequency ( P < 0. 05, P < 0. 01 ) . Compared with the EPM group, the EPM + DZP group showed a significant increase in the distance and number in the central area of the open field test ( P < 0. 05) . Compared with the “—” Maze group, the “—” Maze +Diazepam group ( “—” Maze +DZP ) showed a significant increase of the distance, entry time, and frequency in the central area of the open field test (P<0. 05) . Compared with the EPM group, the EPM+DZP group showed a significant increase in the time and frequency of light-dark box test ( P<0. 05, P<0. 01) . Compared with the “—” Maze group, the “—” Maze+DZP group showed a significant increase in the time and frequency of light-dark box test (P< 0. 05) . Conclusion The open arm entry time and frequency of the “—” Maze are significantly better than those of EPM, and do not have any other effects on animal behavior. It is more suitable for anxiety model testing and is worth further research and development and promotion.




Effects of Parental Folate Deficiency on Body Weight in Offspring Rats

REN Qinghan , ZHANG Guoquan , LI Wen , HUANG Guowei , YAN Jing , LI Zhenshu

2024, 41(6):  11-15.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 003

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Objective The present study was designed to investigate the effect of parental folate deficiency on the body weight of offspring rats. Method The present study was a reproduction experiment in Sprague-Dawley ( SD) rats, where female and male rats were randomly divided into four groups by feeding interventions: parental folate deficient group ( both female and male rats feed folate level <0. 1 mg / kg) ; maternal folate deficient and paternal folate normal group ( female rats feed folate level <0. 1 mg / kg, male rats feed folate level 2. 0 mg / kg) ; maternal folate normal and paternal folate deficient group ( female rats feed folate level 2. 0 mg / kg, male rats feed folate level <0. 1 mg / kg) ; and the group with normal folic acid in both parental generations (2. 0 mg / kg of folic acid in the feed for both female and male rats) . The offspring were weaned at 4 weeks after birth and fed the same diet as their mothers until 13 weeks. The body weight of parental rats, pregnancy rate and prenatal body weight of maternal rats, food intake and body weight of offspring rats were monitored. During the intervention, the body weight of the parental and the offspring rats were recorded. Result The body weight of both parental rats increased with time, and there was no difference in the body weight of the parental among the four groups. The body weight of the offspring increased with time, and the changes of body mass of the offspring rats before and after weaning were lower in D-D group than in the other 3 groups ( P < 0. 05) . 

Conclusion In this study, we haven’ t found folate deficiency could affect the body weight of the parental, while parental folate deficiency may decrease the body weight of the offspring.

Renal Injury in Streptozotocin-induced Diabetic Rats

NIU Yuqi, WANG Jinjin

2024, 41(6):  16-20.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 004

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Objective To establish a diabetic rat model induced by streptozotocin and evaluate its renalinjury. Method

Four-week-old male Sprague-Dawley ( SD) rats were fed a high-fat diet and injected intraperitoneally with streptozotocin ( STZ) at a dose of 30 mg / kg to induce the diabetic model. During the experiment, the general condition of the rats was observed, and fasting blood glucose ( FBG) and body mass was measured. A FBG level ≥ 11. 1 mmol / L was used as the standard for establishing the type 2 diabetes model. After successful modeling, the following parameters were assessed: 24-hour urine protein ( UP ) , urine creatinine ( UCr) , serum creatinine ( SCr ) , and blood urea nitrogen ( BUN) content. Renal tissue was collected to calculate the kidney index (KI) , and HE staining, periodic acid-Schiff ( PAS) staining, and Masson staining were performed to observe renal injury. Result

Compared with the normal group, the model group showed decreased body weight and urine creatinine levels, while 24-hour urine protein, serum creatinine, BUN content, and kidney index were significantly increased ( all P<0. 05) . No obvious abnormalities were observed in the renal pathological staining. Conclusion SD rat model of type 2 diabetes can be established through a 13-week high-fat diet and STZ injection (30 mg / kg) . This model causes renal injury; however, no pathological changes such as glomerulosclerosis, mesangial cell proliferation, or renal fibrosis were observed under light microscopy.

Dynamic Changes and Differentiation Trends of Blood Lipid Levels with Age in Long Clawed Gerbils

ZHAI Ya’ nan , SUN Quan , CHEN Bai’ an , LU Jing

2024, 41(6):  21-26.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 005

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Objective Exploring the changes and differentiation trends of blood lipids in long clawed gerbils of different ages. Method

Select 20 long clawed gerbils each aged 2, 4, 6, 8, 10, 12, 14, and 16 months and feed them with regular rat feed, with half male and half female. After euthanasia of animals, serum was obtained for the detection of four indicators: total cholesterol ( TC) , triglycerides ( TG) , high-density lipoprotein (HDL-C) , and low-density lipoprotein ( LDL-C) . The blood lipid levels of long clawed gerbils at different stages of natural growth and development were compared, and they naturally differentiated into normal blood lipid groups and abnormal blood lipid groups. At the same time, two samples of the largest lobe of the liver were collected, one for histological observation using routine HE staining, and the other for observation of collagen fiber accumulation using Sirius red staining.

Result There were no abnormalities in blood lipids in the 2-month-old and 4-month-old groups, while serum triglycerides ( TG) and total cholesterol ( TC) indicators began to increase in 6-month-old gerbils. From 8-month-old, serum TC levels in animals with dyslipidemia compared to those with normal blood lipids began to increase TG was significantly elevated ( P < 0. 01) , and serum LDL-C was significantly increased in 8-month-old and 16-month-old animals with dyslipidemia compared to animals without dyslipidemia (P<0. 01) . Among animals with abnormal blood lipids, the 8-month-old group accounts for only 20%, while the proportions of 10, 12, and 14-month-old do not exceed 50%. The proportion of animals in the 16-month-old group has reached 70%, and there are more male animals. Pathological examination showed that the liver metabolism of the 2-month-old group of animals was vigorous, with binuclear liver cell division and no other abnormalities found. Fat vacuoles began to appear in the 8- month-old animals, and a large number of liver cells died in the 16-month-old group. The liver cord was unclear, and the hepatic sinusoids were narrowed or even disappeared. Severe steatosis occurred in liver cells, with mild increase in collagen fibers at 14 months old and further significant accumulation of collagen fibers at 16 months old. Conclusion Feeding male long clawed gerbils with regular rat feed up to 16 months old can exhibit typical and stable lipid abnormalities, providing basic support for the study of replicating self occurring hyperlipidemia animal models with similar lesions to humans.

Construction and Phenotypic Analysis of Mutant Mice with Partial Deletion of MIWI C-terminal and 3’UTR

LU Yue, XU Yujun, ZHAO Tingting

2024, 41(6):  27-33.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 006

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Objective To construct mutant mouse with deletion of MIWI protein C-terminal and 3’UTR, and to investigate the effect of partial deletion on the function of MIWI protein. Method Mutant mice with partial deletion of MIWI protein was constructed by CRISPR / Cas9 gene editing technology. Firstly, fertility test was conducted to determine whether the mutation would lead to infertility in male mouse. Secondly, HE staining and other histological method were used to determine the time point of sperm development defects in the male mouse. Finally, the molecular defect of the mutant mice was investigated by Western blot and immunofluorescence staining. Result Fertility test showed that the Miwi + / Del2 heterozygous male mouse was completely infertile, sperm count showed that the total number of sperm decreased by 63%, Western blot showed that the MIWI protein decreased significantly. HE staining of the mutant testis sections showed that round spermatids were abnormal, with significantly increased abnormal sperm ( 74%) . Western blot and immunofluorescence staining results showed that protamine decreased significantly in testis, suggesting that protamine production and / or replacement were affected during sperm deformation, and normal long sperm could not be produced. Conclusion

A Miwi + / Del2 heterozygous mouse with partial deletion of Miwi C-terminal and 3’ UTR was successfully constructed. Deletion of C-terminal of MIWI protein can lead to male sterility.

Industrial Waste Residue Study on Respiratory System Toxicity Effect of Aluminum Ash Extracts in Rats

LIU Lan , MIN Zhe , LI Ruina , WU Guotai , SUN Yingze

2024, 41(6):  34-40.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 007

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Objective Aluminum ash residue extract (AAE) and constructed a rat lung inhalation model to test the effects of AAE on the lungs. Method The physiological and biochemical characteristics of the lungs, as well as the degree of inflammation in the lungs, were examined for different doses and dyeing times of AAE. Result The experimental result showed that nebulized inhalation of AAE could lead to lung injury in rats within a short period of time, even at low doses, and the degree of injury was significantly positively correlated with the inhalation dose and time. The experimental result further indicated that the lung injury caused by the inhalation of AAE was mainly due to the activation of lung neutrophils by AAE, which promoted the release of more pro-inflammatory factors TNF-α, IL-6, IL-1β, and inflammatory mediators MPO and MDA. Conclusion The result of this study reveal the possibility and potential mechanism of industrial solid waste aluminum ash to respiratory tract injury, and provide a reference for promoting the standardized treatment of industrial waste in aluminum manufacturing enterprises.

Elimination of SIRT3 Alleviates Collagen-induced Rheumatoid Arthritis in Rat Model

GAO Ping , ZHAO Liwei , LI Ruisheng , LI Xiaojuan , LI Aimin

2024, 41(6):  41-46.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 008

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Objective The rat model of rheumatoid arthritis was established by using CRISPR technology to knockout SIRT3, and the effects on immune disorder and cartilage damage were analyzed. Method The experiment was divided into WT group ( wild type) , KO group ( SIRT3 was knockout by CRISPR technology) , WT-RA group (wild type rat model of rheumatoid arthritis) , KO-RA group ( a rat model of rheumatoid arthritis in which SIRT3 was knocked out by CRISPR) . The thymus index and spleen of rats were detected. Protein expression of SIRT3, MMP-2, MMP-3, and MMP-9 were detected by Western blot, and the percentage of CD4 + / T lymphocytes and CD8 + / T lymphocytes was analyzed by flow cytometry. The serum cytokines of Th1 ( TNF-α, IFN-γ) and Th2 ( IL-4, IL-10 ) were detected by ELISA. Result Compared with WT group, the levels of SIRT3, CD4 + , CD4 + / CD8 + , thymus index, spleen index, TNF-α, IFN-γ, MMP-2, MMP-3 and MMP-9 in WT-RA group were significantly increased, while the levels of CD8 + , IL-4 and IL-10 were significantly decreased (P<0. 05) . Compared with KO group, the levels of CD4 + , CD4 + / CD8 + , thymus index, spleen index, TNF-α, IFN-γ, MMP- 2, MMP-3 and MMP-9 in KO-RA group were significantly increased, while the levels of CD8 + , IL-4 and IL-10 were significantly decreased ( P < 0. 05 ) . Compared with WT-RA group, the levels of SIRT3, CD4 + , CD4 + / CD8 + , thymus index, spleen index, TNF-α, IFN-γ, MMP-2, MMP-3 and MMP-9 in KO- RA group were significantly decreased, while the levels of CD8 + , IL-4 and IL-10 were significantly increased (P<0. 05) . Conclusion Using CRISPR technology to knock out SIRT3 alleviated the immune disorder and cartilage damage in rheumatoid arthritis rats induced by collagen.

High-fat Diet was Used to Establish the Model of Blood Hypercoagulability in Mice

CHEN Wenxuan , LUO Xiaoying , QUAN Zhihao , HU Xuehan , HU Jing

2024, 41(6):  47-51.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 009

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Objective To explore the feasibility of high-fat diet feeding KM mice to cause hypercoagulable state of blood in mice, and to provide a suitable and simple animal modeling method for the study of hypercoagulable state. Method KM mice were randomly divided into two groups: control group and experimental group, Control group was fed normal diet ( maintenance diet ) and the experimental group was fed high fat diet ( fat for energy 60. 65%) . On the 30th day, the body weight of the mice was measured and the tail venous blood was collected to determine the activated partial thromboplastin time (APTT ) and prothrombin time ( PT ) of the mice. By comparing the differences in blood coagulation indexes between the control group and the experimental group, it was determined whether high-fat diet feeding could cause the occurrence of hypercoagulable state in mice. Result Under the same feeding conditions and blood collection and hemostasis method, compared with control group, mice in the experimental group were fed a high-fat diet for 30 days, the body weight of mice in high-fat diet group was significantly incread ( P < 0. 001) , and PT and APTT were significantly decreased ( P < 0. 01 ) , with statistical significance, and the blood of mice in high-fat diet group showed hypercoagulability. Conclusion The hypercoagulable state model of mice with shortened PT and APTT can be successfully constructed by high-fat diet.

Establishment and Application of Real-time Quantitative PCR Method for Poliovirus Sabin Strain

LIU Susu , YANG Yuansong , WU Yong , ZHAO Haoyang , WANG Xiyan , LIANG Chunnan , FAN Changfa

2024, 41(6):  52-58.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 010

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Objective To establish a real-time quantitative PCR method for poliovirus and detection the viral load of different tissues in Transgenic mice susceptible to poliovirus. Method Poliovirus includes three distinct serotypes named types 1, 2 and 3. First, Primers were designed targeting the VP1 coding regions of three serotypes, and then construct plasmids after PCR identification and sequencing analysis. Making a standard curve by diluting the plasmid copy concentration ten-time series, and evaluated for the specificity, sensitivity, and repeatability of the standard curve. Meanwhile, detection of viral load in infected mouse tissues using established real-time quantitative PCR method method. Result PCR identification and sequencing analysis showed that the sequence of the standard plasmid was complete and correct. The standard curve established has a good linear relationship between copy number and Ct value,with a R value >0. 99. The amplification efficiency is between 99% and 110%, and the minimum detection limit was 10 copy per μL, which is 100 times higher than that of regular PCR. The amplification curve of fluorescence quantitative PCR reaction is S-shaped and the main peak of the dissolution curve is single, indicating good specificity and the amplification conditions were reliable. Applying this method to detect the viral load in various tissues of the infected mouse model, it was found that viral RNA was exclusively detected in the spinal cord and brain tissues, revealing of poliovirus corresponding to the neurotoxicity in monkeys. Conclusion The established real-time quantitative PCR method for poliovirus has good specificity, sensitivity, and repeatability, and can provide a reliable experimental method for quantification of viral load in different tissues in the polio virus permissive mouse

model.


Measurement of Body Growth Curve and Correlation Analysis with Food and Water Intake of British-shorthair Cats in the Laboratory

GAO Anchong , CHEN Jian , LIANG Shuang , YUAN Bao

2024, 41(6):  59-63.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 011

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Objective To study the growth and development patterns of British-shorthair Cats in the

laboratory. Method Determine the growth performance indicators of laboratory cats aged 0 - 24 weeks, including body weight, average daily gain, daily food intake, and daily water intake. Result the body weight of males was higher than that of females at the same age group, with significant differences ( P< 0. 05) between 10 weeks old, 12 - 14 weeks old, 16 weeks old, 19 - 21 weeks old, and no significant differences in other stages; The weight growth rate of laboratory cats at different growth stages is not stable, and the average daily weight gain changes significantly. However, compared with different genders, the difference between the two is not significant in most growth stages, only 5-6 weeks old and 18-19 weeks old. The average daily weight gain of males is significantly higher than that of females (P< 0. 05) . At the age of 0-24 weeks, the daily average feed intake of males was higher than that of females, with significant differences ( P < 0. 05) among the ages of 11 - 12 weeks, 12 - 13 weeks, and 18 - 19 weeks, while no significant differences were observed among other ages. Comparing the daily average water consumption among different genders at the same age group, females aged 11 - 12 weeks were significantly higher than males (P<0. 01) , males aged 12-13 weeks and 17-18 weeks were significantly higher than females ( P < 0. 05) , males aged 19 - 20 weeks were significantly higher than females ( P < 0. 01) , and males in other growth stages were also higher than females, but the difference was not significant. Conclusion The weight of British-shorthair cats aged 0-24 weeks in the laboratory increases with age, and during this period, the weight of males at the same growth stage is consistently higher than that of females. After 12 weeks, there is a significant weight difference between males and females. The turning point of weight gain for male kittens is between 18-19 weeks of age, while for females it is around 21- 22 weeks of age; male kittens have stronger resistance to stress during weaning and separation compared to female cats.

Analysis of Biological Mechanism of Postpartum Rat Model Based on RNA-Seq

LI Ke , LI Yifan , ZHANG Haolin , GUAN Qingye

2024, 41(6):  64-74.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 012

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Objective To analyze the differential expression of genes in postpartum rat animal model by RNA-Seq and characteristics of biological mechanism. Method 9 10-week-old female SD rats were divided into Y0, Y1 and Y2 group randomly, with 3 in each group. RNA was extracted from the abdominal aorta blood of postpartum rats, and transcriptome sequencing was performed using RNA Seq technology. The differentially screened genes were subjected to GO and KEGG bioinformatics analysis. Result Compared Y0, there were 1 222 significantly different genes in both Y1 and Y2. GO analysis showed that significantly different genes were mainly expressed in biological processes related to immunity, oxidative stress and metabolism. KEGG analysis showed that different genes were mainly related to the signal pathways of immune system, signal transduction, signal molecules and interactions, cell growth and death, transportation and catabolism. Conclusion Postpartum rat could simulate the changes of biological function of human postpartum condition as an animal model, could used to observe the impact of pregnancy and childbirth on biological function of the body and explore effective intervention measures.

Characteristics of Gut Microbiota in Representative Rat Strains via 16S rDNA Sequencing Technology

ZHANG Leying, LIU Wenxia, DONG Hao, ZUO Qin

2024, 41(6):  75-81.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 013

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Objective 16S rDNA sequencing was used to study on the characteristics of gut microbiota in four representative rat strains. Method The samples of fresh feces were collected from 48 SD, Wistar, F344, and BN rat strains were collected from the same feeding facility, with half male and half female. The V4 region was amplified with 16S rDNA universal primers. The composition and diversity was analyzed with QIIME. The LEfSe software was used to compare major flora with statistical differences in each strains and gender. Result Bacteroidetes and Firmicutes constitute the main intestinal flora of the rats. Muribaculaceae,Bacteroidesare the main intestinal flora on genera level. The Alpha diversity showed that compared with inbred rats, the outbred rats had higher diversity and richness with higher dispersion. Beta diversity showed that the structure of the gut microbiota from different strains and different genders are different. Conclusion The characteristics of gut microbiota of representative rat strains from National Rodent Laboratory Animal Resources Center is analyzed to rich date support for the related research, which also showed that main intestinal flora from the same breeding condition is similar, and the diversity and richness of the gut microbiotais related to strain and gender.

Construction and Breeding of C3 Gene Over-expression Model Mice

PENG Boya , CHI Yafei , LI Meng , WU Na, CHEN Bai’ an, WU Yi, LU Jing

2024, 41(6):  82-86.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 014

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Objective C3 gene over-expression mice were constructed and bred to identify and analyse their genotypes and the expression of target proteins, and to verify the applicability of the assay. Method The constructed C3 gene over-expression positive founder mice were bred with wild-type mice, and the heterozygous mice were obtained by sibling mating ( male-to-female ratio of 1 ∶ 1 ) , and the pure heterozygous mice were obtained. The genotypes of heterozygotes and heterozygotes were identified by PCR of mouse tail tissue DNA, and the differences in C3 protein expression in brain tissue between mice were detected by Western blot. The body weight changes and reproductive performance of heterozygous and pure heterozygous C3 over-expressing mice were analyzed. Result C3 gene over-expression heterozygous mice could obtain pure heterozygous mice by sibling mating. Protein blotting showed that C3 protein was highly expressed in the tissues of transgenic mice compared with wild-type mice, and the over-expression of C3 gene did not affect the reproduction of mice, and the mating was stable among the homozygous mice. However, over-expression of the C3 gene significantly reduced the body weight of transgenic female mice after 5 weeks of age. Conclusion The C3 over-expression mice can be stably obtained by full-sib mating and genotyping by PCR.

Analysis of Papers Published in the Journal of Laboratory Animal Science from 2007 to 2024 (First Three Issues)

HE Zhengming, CHEN Zhenwen, CHEN Hua, SUN Yansong , CHEN Hongyan , LI Ge , LI Jiping , HU Jianwu

2024, 41(6):  87-96.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 015

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Based on the statistics of 1 914 papers published in 105 issues ofthe Journal of laboratory animal science from 2007 to 2024( first three issues) , the quantitative analysis of the basic information of published papers ( amount of papers, fund project papers, authors, organization distribution, regional distribution, cooperation degree and co-authorship rate, etc. ) and the paper content ( research direction and research hotspot) is performed in this paper. The purpose of this article is to explore the role of this journal, and to understand and grasp the status and trend in the discipline development of laboratory animal science, and also to provide reference for researchers to build academic exchange and dialogue platform in the future.

Research Progress on Rat Models of Recurrent Aphthous Ulcer

LIU Changyue , WANG Zhongke , LIU Ying

2024, 41(6):  97-101.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 016

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Recurrent aphthous ulcer is the most common oral mucosal disease. Because of its unknown etiology, the treatment is mainly to relieve pain, reduce the number of recurrences, and prolong the interval period. The establishment of a good animal model is essential to study the etiology and treatment of recurrent aphthous ulcers. At present, the method for the establishment of recurrent aphthous ulcers include mechanicalmethod, This article reviews several common methods for establishing models and evaluating the results of recurrent aphthous ulcers, and compares the advantages and disadvantages of the models. The aim is to provide a basis for researchers to select models for studying the pathogenesis and drug treatment of recurrent aphthous ulcers.

Trend of Development Analysis and the Role of Toys in the Laboratory Animals Welfare

GENG Xiangfei , WANG Peng , ZHAO Huan , WANG Shuxin

2024, 41(6):  102-105.  DOI: 10. 3969 / j. issn. 1006-6179. 2024. 06. 017

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Enrichment toys for lab animals play an important role in the laboratory animal welfare, and they are also important conditions to ensure the accuracy and reproducibility of animal experiments. As an essential part of the welfare of laboratory animals, toys can provide stimulation and entertainment for laboratory animals, reduce their stress and anxiety, improve their life quality, and express their nature. Because enrichment toys are tools designed to improve the welfare of laboratory animals and promote their nature behavior, so their types, design principles, effects and application prospects have attracted much attention. This article will introduce the laboratory animal welfare related toys and provide reference and help for the development and use of laboratory animal welfare.