Table of Content

28 June 2023, Volume 40 Issue 3

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Urgency and Implementation Path of Popularizing Laboratory Animal Science in China

LIU Ziliang, HU Jianwu

2023, 40(3):  1-6.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 001

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The development of laboratory animal technology is an important indicator of the scientific research level of a country or research unit. As a “ living precision instrument” , laboratory animals have played an important role in national biosafety, biomedical innovation, and even the development of life sciences. Due to various reasons, the scientific popularization of laboratory animals failed to get effective publicity in the early years. However, in recent years, since the prevention and treatment of major infectious diseases such as SARS and COVID - 19 and the technological breakthrough of vaccines, laboratory animals have gradually been recognized by more ordinary people, and the understanding of laboratory animal science has reached a new height. The scientific popularization of laboratory animals and the scientific research of laboratory animals have received unprecedented attention, The scientific popularization of laboratory animals has also received unprecedented demand.


Effect of a High-fat and High-sucrose Diet on the Intestinal Microbiota of Bama Miniature Pigs

NIU Miaomiao, ZHAO Yuqiong, XIANG Lei, JIA Yunxiao, DAI Xin, CHEN Hua

2023, 40(3):  7-11.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 002

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Objective To explore the effect of a high-fat and high-sucrose diet on the blood lipid and gut microbiota of the Bama miniature pigs. Method A total of 10 Bama miniature pigs were selected and randomly divided into control group ( n = 3) and experimental group ( n = 7) . Animals in control group were fed with basic diet and in experimental group with high-fat and high-sucrose diet. Fasting blood lipids, including triglyceride, total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol, were detected before the experiment and in the 8th month. Meanwhile, 16S rRNA sequencing was performed to detect the composition of gut microbiota. Result After 8 months of feeding with high-fat and high-sucrose diet, the number of gut microbiota did not change significantly, however, the relative abundance of Firmicute increased, while the abundance of Bacteroide and Proteobacteria decreased in the experimental group (P<0. 05) . Moreover, the body weight and the level of triglyceride, cholesterol and low-density lipoprotein cholesterol of the experimental group were significantly higher than those of the control group ( P< 0. 05) . Conclusion High-fat and high-sucrose diet could induce the increasement of Firmicute, and the reduction of Bacteroide and Proteobacteria in the feces of Bama miniature pigs, suggesting that the imbalance of these gut microbiota may be involved in regulating the occurrence of hyperlipidemia in the host.


Breeding and Genotype Identification of RELM-β Gene Knockout Rats

CHEN Huilian, ZHOU Lingling, XIE Silin , ZHANG Chao , TAN Junlan , WANG Feiying , YI Jian, DAI Aiguo

2023, 40(3):  12-17.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 003

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Objective To investigate the optimal breeding method and genotype identification method of RELM-β gene knockout rats. Method The RELM-β - / - homozygous female Sprague Dawley ( SD) rats and wild-type male Wild type (WT) SD rats were mated according to 2 ∶ 1 to produce F1 heterozygous rats. Four mating method, RELM-β - / - homozygous cross, RELM-β + / - heterozygous cross, homozygous and heterozygous cross ( orthogonal and reverse cross) , were used to identify the genotype of offspring rats by PCR amplification gel electrophoresis. The appearance of the offspring rats was observed, and the number, weight and homozygous rate of the offspring rats were calculated. Result The genotypes of the parents and offspring rats were successfully identified by PCR amplification gel electrophoresis. There was no significant difference in the appearance and morphology of the three genotypes of rats, no significant difference in the body weight of the rats aged from 3 weeks to 6 weeks, and no significant difference in the number of offspring of different mating method. Conclusion Correct reproduction and identification is an important way to obtain RELM-β gene knockout rats. PCR amplification gel electrophoresis is rapid, economical and reproducible in identifying rat genotypes.


Analysis of Biochemical Parameters of Transgenic Mice Expressing Human Dipeptidyl Peptidase 4 ( hDPP4)

WEI Heru , ZHAI Yueyi , LIU Chao , BAI Tianyu , LIU Shufeng , XIE Ying

2023, 40(3):  18-23.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 004

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Objective In order to provide basic data for the application of MERS-CoV studies, biochemical parameters of transgenic mice expressing human dipeptidyl peptidase 4 were examined. Method A transgenic mouse model that expressing hDPP4, named C57BL / 6J-TgN ( UBC-hDPP4- GFP) CLARS was constructed, which was referred to Tg^hDPP4. Ten blood biochemical indexes, 18 blood physiological indexes, growth and development indexes from 4 to 16 weeks of age and 8 organ indexes of Tg^hDPP4 mice were measured, and compared with wild-type C57BL / 6J mice. Result The alanine aminotransferase and alkaline phosphatase of female Tg^hDPP4 F3 mice was higher than wild-type C57BL / 6J mice. The alanine aminotransferase and alkaline phosphatase of male transgenic mice were lower than the wild-type C57BL / 6J mice. Elevated blood glucose and blood urea ammonia were found in male Tg^hDPP4 F3 mice. Conclusion Through the analysis of the blood biochemical parameters of Tg^hDPP4 F3 mice, it is found that the exogenous hDPP4 gene can affect the blood sugar, blood urea ammonia, alanine aminotransferase and alkaline phosphatase in transgenic mice and may affect the average body weight of Tg^hDPP4 mice.


Application of Nested PCR with HRM in Genotyping Detection of Point Mutation Model Mice

DIAO Ge, TIAN Min, LI Runbo, GUO Jianxin, HAN Jian

2023, 40(3):  24-30.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 005

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Objective Applying nested PCR with high resolution melting ( HRM) technology to improve the genotyping detection method of point mutation model mice. Method Taking ob / ob mice and db / db mice as samples, the mouse toes were cut for numbering and used for extraction of genomic DNAs. Nested PCR primers were designed according to the reference sequence of the mutation site. After two rounds of PCR amplification, the products were directly detected under HRM analysis to obtain genotyping result. The accuracy of the genotyping result was verified by PCR-SBT and PCR-RFLP. Result 80 ob / ob mice and 65 db / db mice were tested, and the nested PCR with HRM method could accurately distinguish the three genotypes. Among ob / ob mice, 21, 47 and 12 were identified as homozygotes, heterozygotes and wild-type mice, respectively. In db / db mice, the numbers of homozygotes, heterozygotes and wild-type were 23, 33 and 9, respectively. The genotyping result of the nested PCR with HRM method were highly consistent with the result of PCR-SBT and PCR-RFLP. Besides, the genotyping result were consistent with the phenotypes of mice. Conclusion The established nested PCR with HRM method is a fast, simple to perform and highly accurate genotyping method, which is suitable for the genotyping of a large number of point mutation model mice.

Reevaluation of Non-clinical Safety of Pudilan Anti-inflammatory Oral liquid

ZHANG Yanhua , JIANG Zhihuan , SHEN Li , LI Chao

2023, 40(3):  31-37.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 006

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Objective The non-clinical safety of Pudilan anti-inflammatory oral solution was reevaluated to provide reference for clinical application. Method Single dose toxicity: Forty SD rats were randomly divided into negative control group, 40. 04, 138. 60 and 295. 56 g / kg groups. Toxicity changes such as body weight and food intake were observed within 14 days after single administration. The 28-day dose toxicity: 160 SD rats were randomly divided into negative control group and 10. 01、32. 03、64. 06 g / kg groups. All rats were administered intragastrically once a day for 28 days with 4-weeks recovery phase. Test indicators included body weight, food intake, ophthalmology, clinical examination, histopathological and so on. Result Single dose toxicity: The 138. 60 g / kg group showed reduced activity and prone lying, and 7 / 10 animals died in the 295. 56 g / kg group. The maximal tolerance dose (MTD) of Pudilan anti-inflammatory oral solution was 138. 60 g / kg after a single administration. The 28-day dose toxicity: Except for hepatocyte hypertrophy in some animals of 32. 03 and 64. 06 g / kg groups, no abnormal changes related to drugs were observed in other groups. The no observed adverse effect level (NOAEL) was 64. 06 g / kg after 28 days of repeated administration. Conclusion The single dose toxicity and 28- day dose toxicity of SD rats showed that Pudilan anti-inflammatory oral liquid was safe and non-toxic in clinical administration cycle, which provided important reference information for clinical administration.


Genetic Polymorphisms of 19 STR Loci in Pembroke Welsh Corgi

YANG Jinlong, XIONG Xin, LI Jinlong

2023, 40(3):  38-41.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 007

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Objective To explore the genetic polymorphisms of 19 STR loci of the Pembroke Welsh Corgi (Hereinafter referred to as corgi) . Method The PCR amplification was performed using the 19 STR fluorescent multiple amplification kit for dog. The amplified products were detected and statistically analyzed. Result To analysis the genetic polymorphisms of 19 loci of 212 purebred corgis, using HardyWeinberg equilibrium testing, all loci had P values greater than 0. 05 except PEZ20,the Cumulative PE (CPE) is 0. 9998289783352165 and the Total Power of Discrimination ( TDP ) is 0. 999999999999998 by 18 STR loci, the average Heterozygosity is 0. 631 and the average Polymorphism Information Content is 0. 604. Conclusion 18 of the 19 STR loci ( except PEZ20 ) can be applied to individual discrimination and paternity test of corgi.


Analysis of Gene Expression Characteristics and Tumor Prognostic Markers in Canine Breast Cancer

TAN Jieren , WANG Xiaoming , CHENG Xu , SHE Zhigang , ZHANG Xiaojing , LI Hongliang , ZHANG Ejuan

2023, 40(3):  42-52.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 008

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Objective To explore the gene expression characteristics and prognostic markers of canine spontaneous breast cancer. Method Multiple canine breast cancer transcriptome datasets were obtained from public databases and corrected and merged to obtain an integrated dataset with large sample size. By bioinformatics analysis, genes related to disease prognosis were clustered. The key characteristic genes that were related to poor prognosis were screened and then verified by cell proliferation assays. Result Cluster 3 obtained by consensus clustering analysis was associated with a low survival rate of the dogs with breast cancer. The characteristic genes in cluster 3 were mainly enriched in cell proliferation-related signaling pathways. By univariate analysis followed by extracting the intersection genes with the human database, 11 cross-species conserved key genes related to poor prognosis of breast cancer were obtained. Two of these conserved genes, ZBTB16 and ABI3BP, were confirmed to be participate in regulating the proliferation of canine breast cancer cells. Conclusion A series of conserved risk genes was obtained. This key gene cluster could be used as biomarkers for disease classification and prognosis evaluation of canine spontaneous breast cancer, as well as the canine breast cancer animal models.


Comparative Study on the Characteristics of Tau Lesions in the Brain of rTg4510 Transgenic Mice and Alzheimer’ s Disease Patients

LI Meng , CHEN Bai’ an , LU Jing

2023, 40(3):  53-60.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 009

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Objective This study aims to provide reference and theoretical basis for selecting rTg4510 transgenic mice for the study of phosphorylated Tau protein metabolism, AD related pathogenic mechanisms, and drug screening. Method The phosphorylated tau protein, amyloid beta( Aβ) plaque and microglia in the brain of rTg4510 transgenic mice and AD patients were detected by immunofluorescence staining with antibodies ( S396, 4G8, IBA1 ) , software ( CaseViewer, IPWIN Application, and GraphPad Prism 8) were used to analyze the results, the expression, distribution and type of phosphorylated tau protein aggregation in the brain of rTg4510 transgenic mice and AD patients, as well as the characteristics of microglia lesions and Aβ plaques were compared. Result Similar to the characteristics that the expression of phosphorylated Tau protein ( S396 ) in cerebral cortex and hippocampus of AD patients was significantly higher than that of healthy control group ( p<0. 001) , the expression of phosphorylated Tau protein ( S396 ) in cerebral cortex and hippocampus of rTg4510 transgenic mice was significantly higher than that of wild-type control mice ( p< 0. 001) . Similar to the characteristics that the expression of phosphorylated Tau protein ( S396) in cerebral cortex of AD patients was significantly higher than that in hippocampus ( P < 0. 001) , the expression of phosphorylated Tau protein ( s396) in the cerebral cortex of rTg4510 transgenic mice has a similar trend compared with the expression of phosphorylated Tau protein ( s396) in the hippocampus. Similar to AD patients, type 1 and type 2 aggregation of hyperphosphorylated tau protein ( S396 ) were found in the brain of rTg4510 transgenic mice. Similar to AD patients, rTg4510 transgenic mice brain microglia ( Iba1) are sensitive to the recognition of hyperphosphorylated Tau protein ( S396) . Different from the abnormal expression of Aβ plaques (4G8) associated with hyperphosphorylated Tau protein ( S396) in the brain of AD patients, the abnormal expression of Aβ plaques ( 4G8 ) was not associated with hyperphosphorylated Tau protein ( S396) in the brain of rTg4510 transgenic mice. Conclusion rTg4510 transgenic mice will be a useful animal model for studying the pathogenic mechanism of AD related phosphorylated Tau protein and drug development.


Experimental Study on Animal Models of Spontaneously Persistent Atrial Fibrillation

ZHANG Dong , ZHANG Min , ZHU Jinyan , LI Bin

2023, 40(3):  61-70.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 010

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Objective To establish an animal model of spontaneous persistent atrial fibrillation. Method Guizhou miniature pigs were selected and the mitral regurgitation and sham operation group models were established after anesthesia induction and respiratory intubation. Mitral regurgitation model group ( 4 cases) and sham operation group ( 4 cases ) were compared to observe the occurrence and development of spontaneous persistent atrial fibrillation within the longest postoperative period ( 6 months) . During the period ( before surgery, immediately after surgery, 3 months after surgery, 6 months after surgery ) , electrocardiogram and cardiac ultrasound were performed respectively, so as to clarify the situation of atrial fibrillation and valve regurgitation. If spontaneous persistent atrial fibrillation appeared in the model during the period, the experiment was immediately terminated and three-dimensional electrophysiological mapping was performed. If there was no spontaneous persistent atrial fibrillation in the experimental animal model, the longest observation time was 6 months, and three-dimensional electrophysiological mapping and susceptibility test of atrial fibrillation were performed. Result The model of mitral regurgitation and sham operation group was established successfully. No spontaneous persistent atrial fibrillation occurred in the longest period ( 6 months) of each group. In the mitral regurgitation group, the left atrium was obviously enlarged and showed obvious structural remodeling. Compared with the control group, the fraction of short atrial fibrillation in the mitral regurgitation group was significantly reduced, and the ratio of heart rate to total heart rate of atrial fibrillation after pacing stimulation was significantly increased. The mitral regurgitation group was significantly more susceptible to atrial fibrillation than the control group. Conclusion An animal model of persistent atrial fibrillation was successfully established in this study, and it was found that mitral regentation can significantly increase the susceptibility to atrial fibrillation.


Detection Capacity of Peptidase 3 in the Proficiency Testing of Laboratory Animals

WANG Hong, WEI Jie, LI Huan, ZHOU Jiaqi, YUE Bingfei, MA Liying

2023, 40(3):  71-74.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 011

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Objective To evaluate the capacity of the laboratories of quality control for laboratory animals and improve the level of quality control of laboratories through the proficiency testing. Method To carry out the proficiency testing according to the document CNAS-CL03, and samples passed the homogeneity test and stability test. The participating laboratories should submit the testing result and original record. The laboratory result were compared with the standard result. Result A total of 8 laboratories participated in the program. 6 laboratories presented satisfactory experiment result. The satisfaction rate of the program is 75%. Conclusion The overall testing level of peptidase 3 in the laboratories of quality control was relatively high. There is room for improvement in a few laboratories.


Effect of Mixed Ratio of Zoletil 50 and Sumianxin II on Anesthesia in SPF Mice

NIE Yongsheng , CHEN Xi , LIANG Pingping , WU Qian , RAN Qian, LIN Xinwen , PAN Yuhui, ZENG Lin , LIU Mingming , WANG Wei

2023, 40(3):  75-79.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 012

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Objective To explore the anesthesia effect of the new uncontrolled anesthetic drug Zoletil 50 and Sumianxin Ⅱ on SPF mice, hoping to find out a method that can achieve the ideal anesthesia effect for all strains of mice. Method Referring to the instructions of the drug, taking the dosage of dogs, cats and rabbits as reference, the mice of each strain were administered by intraperitoneal injection by using Sumianxin 11 ( 30-fold dilution) alone; Zoletil 50 ( 10-fold dilution) alone; mixed Sumianxin 11 and Sutai 50 both diluted by 10 times with 1 ∶ 1 mixing ratio; and mixed Sumianxin 11 ( 20 times dilution) and Zoletil 50 ( 5 times dilution ) 1 ∶ 1 mixing ratio, erspectively, and the administration dose was 0. 1 mL / 20 g. Five CD-1 strains were used to administer the single drug, and six strains were used to administer the mixed drug, with 10 mice in each group. Result All the mice with Sumianxin 11 died, and the mice with Zoletil 50 alone could not reach the surgical requirement of anesthesia. In both groups of the mixed Sumianxin 11 and Sutai 50, except for one mouse that died due to improper insulation, the rest of the mice could reach the requirement of surgical anesthesia and wake up normally. Conclusion Converting the result to the working concentration, SuMianxin Ⅱ with the concentration of 10-25 mg / kg combined with the concentration of 10 - 25 mg / kg of Sutai 50 at a ratio of 1 ∶ 1 can achieve the ideal effect in the actual use of intraperitoneal injection anesthesia in mice, suggesting a strong application value in mouse experimental anesthesia.


Standardized Control of Animal Experiment Quality and Interpretation of the New Local Standards in Beijing

LI Nan, WANG Tianqi, ZHANG Changyong, SUN Xizhen, BAO Guo, HE Jialing, SUN Deming

2023, 40(3):  80-83.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 013

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Animal experiment plays an irreplaceable role in the field of biomedical research. It can quickly promote the rapid development of multi-disciplinary and multi-field related to animal experiment via standardization of quality control of animal experiment technology. This paper the introduces the background and main provisions of animal experiment management and technical specification for Beijing’ s new local standards (DB11 / T 1717—2020) , so that relevant personnel can better understand the standard, and better control the quality standardization of animal experiments. The establishment of this standard could improve the scientific and standardization of animals experiment in order to further promoting the development of science.


Effect of Different Anesthesia Methods on PET / CT Examination Results of Miniature Pigs

LIU Tianwen

2023, 40(3):  84-87.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 014

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Objective To observe the effects of two anesthesia method on the experimental result of myocardial perfusion examination using 18F-Flurpiridaz in miniature pigs ( mini pigs ) . Method 4 normal Bama mini pigs were randomly divided into 2 groups with 2 pigs in each group. Group A was anesthetized by intramuscular injection of Lumianning and midazolam. Group B received Zoletil and midazolam combined muscle anesthesia. The result of myocardial perfusion imaging were compared between two groups. Result False positive myocardial perfusion imaging was found in group A,and as expected in group B. Conclusion Base anesthesia of mini pigs can be achieved by both anesthesia programs. However, it is suggested to avoid or reduce the use of Lumianning for combined anesthesia in cardiovascular related experimental examination.


Analysis on the Detection of Field Mouse Pathogens from 2017 to 2020

ZHANG Wanlin, ZHANG Meng, XIE Yuwei, MIAO Xiangnan, ZHANG Qiaozhi, WANG Xuemei, LUO Liping, HAN Xue

2023, 40(3):  88-91.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 015

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Objective From 2017 to 2020, field mouse or related samples around facilities were collected and tested for infectious agents by PCR method, in order to analyze the types of pathogens carried by field mouse, improve pest control method and effects, and reduce pathogens risk around facilities. Method Fresh field mousefeces or environmental swabs were collected in the poison bait station field mouse entered. Fur swabs were collected from field mouse captured by mechanical mousetraps or strangulation cages. All samples were sent to Vital River Lab to conduct comprehensive PCR testing. Result Total of 40 samples were collected and tested, and 11 types of bacteria, 3 types of viruses, and 5 types of parasites were founded. Among them, Helicobacter is commonly founded with 75% positive rate. Conclusion Through detection and analysis, the types of pathogens carried by field mouse ( including zoonoses) and the detection rate are high.


Effect of Astragaloside IV on Type 1 Diabetic Retinopathy in Rats

JIANG Fan, SUN Jichao, ZHANG Jia, WU Huijuan

2023, 40(3):  92-97.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 016

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Objective The purpose of this study was to study the prevention and treatment effect of astragaloside IV on type 1 diabetic retinopathy in rats and explore its mechanism. Method The type 1 diabetic rat model was established. The rats whose blood glucose reached the required level were randomly divided into five groups and a normal control group with ten rats in each group. Astragaloside IV was given by intragastric administration to the rats, their blood glucose and body weight were monitored regularly. After 16 weeks, the levels of inflammatory factors( IL-6, TNF-α) in retinal tissues and oxidative stress indexes ( MDA, SOD ) in serum were detected, and the retinal pathology was analyzed. Result Astragaloside IV improved type 1 diabetic retinopathy in rats. Compared with the model control group, astragaloside IV significantly decreased the contents of IL-6 and TNF-α in retinal tissue and MDA in serum (P<0. 05) , increased the activity of SOD in serum (P<0. 05) , and decreased the expression of VEGFA in retina. Conclusion Astragaloside IV has protective effect on type 1 diabetic rat retinopathy, and its mechanism may be related to inhibition of inflammatory factors, oxidative stress level and VEGFA expression.

Research Progress on the Influencing Factors of Fundus Fluorescein Angiography in Experimental Animals

SUN Xiaorong, YANG Diyuan, SU Dan , GUI Wenjuan, CHEN Yue

2023, 40(3):  98-103.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 017

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Fluorescein fundus angiography ( FFA) can accurately and intuitively reflect fundus-related lesions, and is indispensable in the detection of fundus disease animal models. The quality of FFA images is mainly affected by the appearing intensity and appearing time, which are controlled by many factors. The author combines many years of FFA experience in various species of experimental animals and refers to domestic and foreign research literature, summarize and analyze various controllable factors that affect the appearing intensity and time of FFA. We focus on comparing the differences in blood viscosity, blood pH, body temperature of different species of experimental animals and human, as well as the appearing intensity of FFA and the initial time, maintenance time and subside time. The differences of FFA among experimental animal species and the factors causing animal adverse reactions during FFA were analyzed. We hope the article which providing the information and data can help solve the problems encountered by researchers in FFA of experimental animals, obtain more accurate and reliable detection result, and realize the application value of FFA detection in experimental animals.

Research Progress of Surgical Models of Neointimal Hyperplasia and Restenosis

YAO Zhichao, ZHU Yan, HUO Lirong

2023, 40(3):  104-108.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 018

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With the change of life style in modern society, the number of patients with atherosclerosis is increasing in recent years, and the number of patients requiring angioplasty is also increasing significantly. Existing treatment measures have a high incidence of post-operative restenosis. Clarifying the mechanisms of restenosis is of great significance for their prevention and treatment. At present, neointimal hyperplasia and restenosis model has been mature and widely used to simulate the situation of vascular restenosis after angioplasty. At present, there are a variety of arterial injury method to replicate the animal model of neointimal hyperplasia, such as balloon injury, wire injury, ligation, cuff injury. All of these method can cause intima hyperplasia and then lead to vascular stenosis. This paper will review the characteristics of various modeling method to summarize the advantages and disadvantages of each method, in order to find a most practical modeling method and provide references for experimental vascular disease studies.


Research Progress on the Pathological Mechanism of Reproductive Injury in Diabetic Males

XUE Jingwen, MA Xiaoru

2023, 40(3):  109-113.  DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 019

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In recent years, male reproductive disorders caused by diabetes have become the focus of human attention as the global infertilities of young people is increasing in reproductive age. Diabetes is a chronic metabolic syndrome caused by a relatively low insulin secretion that leads to elevated blood glucose. The major vascular complications caused by diabetes include diabetic cardiomyopathy, diabetic nephropathy and retinopathy, among which reproductive function injury is also one of them. The mechanism of diabetic testicular tissue damage involves many aspects, including glucose and lipid metabolism, oxidative stress, inflammatory response, endoplasmic reticulum stress, autophagy and so on. In this review, the pathogenesis of male reproductive disorders induced by diabetes was briefly introduced, which provided a new research idea for further exploring the damage of high glucose on testicular spermatogenic cells, and a new drug target for clinical treatment.