Abstract: Objective To know the quality of laboratory animals,to publicize and carry out the new GB, the pathogen of conventional mice and rats in Shandong province from 2000 to 2002 was checked. Method Conventional mice and rats were choosed from different unit of different regions in Shandong province,then we tested whether they carried the pathogenic bacteria and virus according to the standards of clean laboratory animals. Results The sera positive rates of Mouse Hepatitis Virus and Sendai Virus were 11% and 19% respectively. The sera positive rate of other virus is zero in mouse.The sera positive rates of rat Hanta virus and Sendai virus are zero. The positive rate of Bordetella browchiseptica in rats is 8 3%.But all the other pathogenic bacteria,except Mycoplasma spp. and Tyzzer's organism unchecked,are negative. Conclusion Shandong province had improved obviousely in the quality of laboratory animals,attained the conventional standards.But the existence of MHV and SV in mouse and the hidden infection of Bordetella bronchiseptica in rats suggested that was needed to spread clean mice and rats in Shandong province.

Key words: Mice, Rats, Pathogenic bacteria, Virus

摘要： 目的　通过对山东省大部分单位普通级大、小鼠病原体携带状况的调查 ,了解省内实验动物质量情况 ,以利于新国标的宣传和执行。方法　选取省内不同地区、不同单位的普通级大、小鼠 ,按清洁级标准对其所携带细菌病毒进行检测。结果　小鼠肝炎病毒 (MHV)和小鼠仙台病毒 (SV)血清抗体阳性率分别为 11%和 19%。小鼠淋巴细胞脉络丛脑膜炎病毒 (LCMV)、汉坦病毒 (HV)和鼠痘病毒 (Ect )血清抗体均为阴性。大鼠HV、SV血清抗体均为阴性。细菌检测结果 ,Wistar大鼠支气管鲍特杆菌阳性率为 8 3% ,其余所检项目均为阴性 (支原体、泰泽氏菌未能检测 )。结论　山东省实验动物质量有了明显提高 ,达到了普通级要求 ;但是 ,MHV和SV的存在以及Wistar大鼠支气管鲍特杆菌的隐性感染 ,说明普及清洁级大小鼠是很有必要的

关键词: 小鼠, 大鼠, 病原菌