Abstract: Objective:To study the relationship between inflammatory activities and coagulation abnormal in the TNBS induced rat's colitis model. Method 30 SD-rats separated into 3 groups randomly,Include 1 normal control group: In this group every rats were given an enema with normal saline, and were killed 14 day after enema. 2 1week colitis group, in this group every rat was given an enema with TNBS, and was killed 7 day after enema. Colitis 2 weeks group: In this group every rats were given an enema with TNBS, and were killed 14 day after enema. We choose PT、APTT and the activity of AT as the index of coagulation. And we assess the level of damage and inflammatory state of the colitis rats by macroscopically score, histological score and the level of TNF-α in each group. Result Compared with normal control group, TNBS induced 1 week colitis group, and 2weeks colitis group has a shorter PT and APTT and a lower activity level of AT. The number of plate per litre is higher than the normal control group. The difference has statistical significance. And we find that index of tissue damage and inflammatory has negative correlation with PT 、APTT and AT. Conclusion There is abnormality of coagulation in the TNBS induced rat colitis,which is correspondence with the state of the illness closely. The test's result implied that abnormal state of coagulation in IBD patients have close correlation with the activity of inflammatory. And TNBS induced rat colitis model can act as a model of inflammation activate coagulation for further study in this field.

Key words: Inflammatory bowel disease, TNBS, Colitis model, Hyper-coagulate state, Inflammatory

摘要： 目的探讨炎症性肠病中凝血异常与炎症的关系。方法检测三硝基苯磺酸钠(trinitro-benzene-sulfonic acid TNBS)灌肠诱导大鼠结肠炎模型中的凝血指标明确有无凝血异常。将30只SD大鼠分为三组,(1)正常对照组:仅用生理盐水灌肠。(2)结肠炎1周组,TNBS灌肠1周后处死,模拟结肠炎中的急性炎症期。(3)结肠炎2周组:TNBS灌肠2周后处死,模拟慢性炎症期。处死后取血检查凝血酶原时间(prothrombin time PT)、活化部分凝血激酶时间(activated partial thromboplastin time APTT)、血小板计数以及抗凝血酶活性(antithrombin AT)。处死同时检查组织损伤和炎症水平指标。结果TNBS诱导的结肠炎模型中凝血酶原时间、活化部分凝血激酶时间均较正常对照组为短;抗凝血酶活性较正常对照组下降,血小板数目较对照组升高。差异均有统计学意义(P<0.05)。通过相关分析,组织损伤以及炎症指标和PT、APTT以及抗凝血酶活性成负相关(P<0.01),与血小板计数成正相关。结论TNBS诱导的结肠炎模型中存在凝血异常,且与病情严重程度有相关性。提示临床上的炎症性肠病中存在凝血异常和炎症活动水平关系紧密。本模型可以作为炎症激活凝血异常的模型来研究这一现象。

关键词: 炎症性肠病, 三硝基苯磺酸钠, 结肠炎模型, 高凝状态, 炎症